三种多糖的结构表征及其对鸡巨噬细胞免疫调节活性的比较研究。
Structure characterization of three polysaccharides and a comparative study of their immunomodulatory activities on chicken macrophage.
机构信息
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Zhengzhou Ninth People's Hospital, Zhengzhou 450053, PR China.
出版信息
Carbohydr Polym. 2016 Nov 20;153:631-640. doi: 10.1016/j.carbpol.2016.07.116. Epub 2016 Jul 28.
In this study, we evaluated structure characterization and immunomodulatory activity of polysaccharides from Astragalus aboriginum Richardson (RAPS), Atractylodes macrocephala Koidz (RAMPS) and Rumia seseloides Hoffm (RSPS) in vitro on chicken macrophage. We found that molecular weight of RAPS and RAMPS was 122.4 and 109.4kDa higher than 64.71kDa of RSPS. Glucose occupied 83.95% and 66.39% in RAPS and RAMPS, respectively. RSPS mainly contained glucose and galacturonic acid, which accounted for 32.35% and 29.25%, respectively. The NMR results displayed that RAPS and RAMPS contained β- glucose, β-galactose, and β-galacturonic acid. The backbone was 1→6 linked glucose. RSPS showed at least six monosaccharide response signals. In vitro experiment, the results showed that RAPS at dosage of 15.62μgmL(-1) exhibited significant immunological on chicken macrophage compared to RAMPS and RSPS. Interestingly, costimulatory molecules levels in RSPS group were higher than that of RAPS, which may associated with the special structure of RSPS.
在这项研究中，我们评估了黄芪（RAPS）、白术（RAMPS）和獐牙菜（RSPS）多糖的体外结构特征和免疫调节活性对鸡巨噬细胞的影响。我们发现，RAPS 和 RAMPS 的分子量分别为 122.4 和 109.4 kDa，高于 RSPS 的 64.71 kDa。葡萄糖分别占 RAPS 和 RAMPS 的 83.95%和 66.39%。RSPS 主要含有葡萄糖和半乳糖醛酸，分别占 32.35%和 29.25%。NMR 结果表明，RAPS 和 RAMPS 含有β-葡萄糖、β-半乳糖和β-半乳糖醛酸。其糖苷键为 1→6 连接的葡萄糖。RSPS 至少显示出六种单糖响应信号。体外实验结果表明，与 RAMPS 和 RSPS 相比，剂量为 15.62μgmL(-1)的 RAPS 对鸡巨噬细胞表现出显著的免疫作用。有趣的是，RSPS 组的共刺激分子水平高于 RAPS 组，这可能与 RSPS 的特殊结构有关。
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