Naseh Ghodratolah, Mohammadifard Mahyar, Mohammadifard Mahtab
Department of Surgery, Imam Reza Hospital, Birjand University of Medical Sciences, Birjand, Iran.
Department of Radiology, Imam Reza Hospital, Birjand University of Medical Sciences, Birjand, Iran.
IUBMB Life. 2016 Oct;68(10):799-805. doi: 10.1002/iub.1543. Epub 2016 Aug 25.
The aim of this study was to evaluate the protein and mRNA expressions of matrix metalloproteinase-14 (MMP14) and CDK7 in gastric cancer (GC) tissues. Upregulation of MMP14 mRNA level was observed in GC tissues when compared with the matched normal tissues (mean ± SD: 3.92 ± 1.15 vs. 1.35 ± 0.81, P < 0.001). This study indicated that mRNA levels of CDK7 were statistically overexpressed in GC when compared with matched normal tissues (4.12 ± 0.84 vs. 1.43 ± 0.71, P < 0.001). The protein levels of MMP14 were found to be increased in GC (60.41%; P < 0.001). The expression of CDK7 was higher in GC tissues than matched normal tissues (70.83; P < 0.001). We found that high MMP14 expression was related to advanced TNM stage (P = 0.004), tumor grade (P = 0.002), and lymph node metastasis (P = 0.015), but no association with other clinical variables (P > 0.05). In addition, high expression of CDK7 was significantly linked to advanced TNM stage (P = 0.001), pathological grade (P = 0.012), and presence of lymph node metastasis (P = 0.009), while no correlation between CDK7 expression and other clinical variables, such as age and gender, distance metastasis. The patients with high expression of MMP14 and CDK7 exhibited worse survival time than those with higher levels. Cox multivariate regression analysis clearly showed that high expression of MMP14 and CDK7 was independent prognostic factors for overall survival in patients with GC. Taken together, these results indicated the overexpression of above markers in the progression and the tumorigenesis of GC and overall patient survival. © 2016 IUBMB Life, 68(10):799-805, 2016.
本研究旨在评估基质金属蛋白酶-14（MMP14）和细胞周期蛋白依赖性激酶7（CDK7）在胃癌（GC）组织中的蛋白和mRNA表达。与配对的正常组织相比，GC组织中观察到MMP14 mRNA水平上调（均值±标准差：3.92±1.15 vs. 1.35±0.81，P<0.001）。本研究表明，与配对的正常组织相比，GC组织中CDK7的mRNA水平在统计学上过度表达（4.12±0.84 vs. 1.43±0.71，P<0.001）。发现GC中MMP14的蛋白水平升高（60.41%；P<0.001）。GC组织中CDK7的表达高于配对的正常组织（70.83；P<0.001）。我们发现MMP14高表达与TNM晚期（P=0.004）、肿瘤分级（P=0.002）和淋巴结转移（P=0.015）相关，但与其他临床变量无关联（P>0.05）。此外，CDK7高表达与TNM晚期（P=0.001）、病理分级（P=0.012）和淋巴结转移的存在（P=0.009）显著相关，而CDK7表达与其他临床变量如年龄、性别、远处转移之间无相关性。MMP14和CDK7高表达的患者生存时间比表达水平较低的患者更差。Cox多因素回归分析清楚地表明，MMP14和CDK7高表达是GC患者总生存的独立预后因素。综上所述，这些结果表明上述标志物在GC的进展、肿瘤发生及患者总体生存中过度表达。©2016国际生物化学与分子生物学联盟生命科学，68(10):799 - 805，2016。
