Özalp Burhan, Elbey Hüseyin, Aydın Hülya, Tekkesin Merva S, Uzun Hafize
Department of Plastic, Reconstructive and Aesthetic Surgery, Dicle University Medical Faculty, Diyarbakır, Turkey.
Department of Plastic, Reconstructive and Aesthetic Surgery, Dicle University Medical Faculty, Diyarbakır, Turkey.
J Surg Res. 2016 Aug;204(2):304-310. doi: 10.1016/j.jss.2016.04.075. Epub 2016 May 7.
Coenzyme Q10 (CoQ10) is a lipid-soluble benzoquinone with antioxidant features that make it important in the treatment of ischemia reperfusion injury. In this study, we aimed to investigate the beneficial effect of CoQ10 in the treatment of venous ischemia/reperfusion injury.
Eighteen Sprague-Dawley male rats were randomly divided into two equal groups: the control group and an experimental group (n = 9 rats). The experimental group received CoQ10 orally, and the control group received a control diet for 8 wk. An inferior epigastric island flap was raised, and the inferior epigastric vein was clamped for 9 h; the flap was then reperfused. All rats were sacrificed on postoperative day 5. The flap survival rate and levels of CoQ10, malondialdehyde, glutathione, and superoxide dismutase were assessed, and flap tissues were examined under a light microscope (×200 magnification) after being stained with Hematoxylin & Eosin.
The flap survival rate and levels of CoQ10, glutathione, and superoxide dismutase were significantly higher, but level of malondialdehyde was lower in the experimental group. The mean flap survival rates and plasma levels of CoQ10 were 51% ± 24% and 251 ± 11 ng/mL in the control group, whereas they were 88% ± 7% and 692.8 ± 79.7 ng/mL in the experimental group with statistically significant differences (P < 0.001). Polymorphonuclear leukocyte infiltration was higher, and surface epithelial integrity was more impaired in the control group.
We concluded that CoQ10 supplementation has a beneficial effect on venous ischemia and/or reperfusion injury and improves flap survival rate.
辅酶Q10（CoQ10）是一种具有抗氧化特性的脂溶性苯醌，在缺血再灌注损伤的治疗中具有重要作用。在本研究中，我们旨在探讨CoQ10在静脉缺血/再灌注损伤治疗中的有益作用。
将18只雄性Sprague-Dawley大鼠随机分为两组，每组9只：对照组和实验组。实验组口服CoQ10，对照组给予对照饮食，持续8周。掀起腹壁下岛状皮瓣，夹闭腹壁下静脉9小时；然后进行皮瓣再灌注。所有大鼠于术后第5天处死。评估皮瓣存活率以及CoQ10、丙二醛、谷胱甘肽和超氧化物歧化酶的水平，并用苏木精和伊红染色后在光学显微镜（×200倍放大）下检查皮瓣组织。
实验组的皮瓣存活率以及CoQ10、谷胱甘肽和超氧化物歧化酶水平显著更高，但丙二醛水平更低。对照组的平均皮瓣存活率和血浆CoQ10水平分别为51%±24%和251±11 ng/mL，而实验组分别为88%±7%和692.8±79.7 ng/mL，差异具有统计学意义（P<0.001）。对照组的多形核白细胞浸润更高，表面上皮完整性受损更严重。
我们得出结论，补充CoQ10对静脉缺血和/或再灌注损伤具有有益作用，并可提高皮瓣存活率。
