Hwang Jin-Young, Min Seong-Won, Jeon Young-Tae, Hwang Jung-Won, Park Sang-Heon, Kim Jin-Hee, Han Sung-Hee
Department of Anesthesiology and Pain Medicine, SMG-SNU Boramae Medical Center; and.
J Neurosurg Spine. 2015 Apr;22(4):432-8. doi: 10.3171/2014.12.SPINE14487. Epub 2015 Jan 30.
Spinal cord ischemia remains a serious complication of thoracoabdominal aortic aneurysm surgery. Coenzyme Q10, a potent antioxidant, has been reported to exert a neuroprotective effect. In the present study, we evaluated the effect of coenzyme Q10 pretreatment on spinal cord ischemia-reperfusion injury.
Male Sprague-Dawley rats were treated with either 300 mg/kg coenzyme Q10 (CoQ10 group, n = 12) or saline (control and sham groups, n = 12 for each group) for 5 days before ischemia. Spinal cord ischemia was induced in the control and CoQ10 groups. Neurological function was assessed using the Basso-Beattie-Bresnahan (BBB) motor rating scale until 7 days after reperfusion, and then the spinal cord was harvested for histopathological examinations and an evaluation of malondialdehyde level.
On post-reperfusion Day 1, the CoQ10 group showed higher BBB scores compared with those in the control group, although the difference was not significant. However, on Day 2, the CoQ10 group showed a significantly higher BBB score than the control group (14.0 [10.3-15.0] vs 8.0 [5.0-9.8], median [IQR], respectively; p = 0.021), and this trend was maintained until Day 7 (17.5 [16.0-18.0] vs 9.0 [6.5-12.8], respectively; p < 0.001). Compared with the control group, the CoQ10 group had more normal motor neurons (p = 0.003), fewer apoptotic changes (p = 0.003) and a lower level of tissue malondialdehyde (p = 0.024).
Pretreatment with 300 mg/kg coenzyme Q10 resulted in significantly improved neurological function and preservation of more normal motor neurons.
脊髓缺血仍然是胸腹主动脉瘤手术的严重并发症。辅酶Q10是一种有效的抗氧化剂，据报道具有神经保护作用。在本研究中，我们评估了辅酶Q10预处理对脊髓缺血再灌注损伤的影响。
雄性Sprague-Dawley大鼠在缺血前5天接受300mg/kg辅酶Q10治疗（辅酶Q10组，n = 12）或生理盐水治疗（对照组和假手术组，每组n = 12）。对照组和辅酶Q10组诱导脊髓缺血。使用Basso-Beattie-Bresnahan（BBB）运动评分量表评估神经功能，直至再灌注后7天，然后采集脊髓进行组织病理学检查和丙二醛水平评估。
再灌注后第1天，辅酶Q10组的BBB评分高于对照组，尽管差异不显著。然而，在第2天，辅酶Q10组的BBB评分显著高于对照组（分别为14.0[10.3-15.0]对8.0[5.0-9.8]，中位数[IQR]；p = 0.021），并且这种趋势一直维持到第7天（分别为17.5[16.0-18.0]对9.0[6.5-12.8]；p < 0.001）。与对照组相比，辅酶Q10组有更多正常运动神经元（p = 0.003），凋亡变化更少（p = 0.003），组织丙二醛水平更低（p = 0.024）。
300mg/kg辅酶Q10预处理可显著改善神经功能，并保留更多正常运动神经元。
