Stepanov S I, Semenova E V, Noskin L A, Drobchenko E A, Filatov M V
Tsitologiia. 1989 Apr;31(4):410-8.
The authors discuss various aspects of a recently developed method permitting a detailed flow cytometric analysis of the individual cell karyotypes such as instrumentation, histochemistry, data proceeding algorithms. Possible drawbacks of the method and the ways of their overcoming are considered. Results of analysis of the Chinese hamster cells are presented that illustrate the possibilities of the method, including the metaphase chromosome distribution according to their fluorescence intensity, the analysed cell distribution according to their chromosomes number, the table in which the individual cell karyotypes are distributed according to their fluorescence. The results obtained show that the developed method may be successfully used for investigating chromosomal iNstability and heterogeneity of the mammalian cells.
作者讨论了最近开发的一种方法的各个方面，该方法允许对单个细胞核型进行详细的流式细胞术分析，如仪器设备、组织化学、数据处理算法。还考虑了该方法可能存在的缺点及其克服方法。展示了对中国仓鼠细胞的分析结果，这些结果说明了该方法的可能性，包括中期染色体根据其荧光强度的分布、分析细胞根据其染色体数量的分布，以及单个细胞核型根据其荧光分布的表格。所获得的结果表明，所开发的方法可成功用于研究哺乳动物细胞的染色体不稳定性和异质性。
