Filatov M V, Kotlovanova L V, Stepanov S I, Tret'iakov A N, Strel'tsov P G
Tsitologiia. 1988 Aug;30(8):999-1007.
The karyotypes of individual cells in clones of the established Chinese hamster cell line display a highly heterogeneous pattern. Unlike situation in individual cells, the flow karyotypes of cloned cell populations are very similar. A comparison of these facts suggests that mostly the same certain chromosomal reorganizations, appearing frequently enough, may occur in the cells. As a result, the whole set of possible variants of reorganized chromosomes appear during few cell cycles, regardless of the initial cell karyotype. This hypothesis is supported by our flow cytometry data. The same small peaks corresponding to rarely met (less than 1 per cell) rear ranged chromosomes appear on flow karyotype histograms of parental cell clones and their secondary subclones. Chromosomes with random gamma or UV irradiation-induced reorganizations do not remain in the cell population, unlike certain reorganization of regular nature.
已建立的中国仓鼠细胞系克隆中单个细胞的核型呈现出高度异质的模式。与单个细胞的情况不同，克隆细胞群体的流式核型非常相似。对这些事实的比较表明，细胞中可能大多发生了相同的某些染色体重排，且出现频率足够高。因此，无论初始细胞核型如何，在少数细胞周期内就会出现整套可能的重排染色体变体。我们的流式细胞术数据支持这一假设。在亲代细胞克隆及其二代亚克隆的流式核型直方图上，出现了对应于罕见（每个细胞少于1条）重排染色体的相同小峰。与某些常规性质的重排不同，经随机γ或紫外线照射诱导重排的染色体不会保留在细胞群体中。
