绵羊奶β-乳球蛋白（β-Lg）基因座存在第三个等位基因的证据及其在不同品种中的出现情况。

Evidence for a third allele at the beta-lactoglobulin (beta-Lg) locus of sheep milk and its occurrence in different breeds.

作者信息

Erhardt G

机构信息

Institute of Animal Science, Justus-Liebig-University, Giessen, FRG.

出版信息

Anim Genet. 1989;20(2):197-204. doi: 10.1111/j.1365-2052.1989.tb00857.x.

DOI:10.1111/j.1365-2052.1989.tb00857.x

PMID:2757271

Abstract

Milk samples from 189 Merinoland Sheep, 145 Black Faced Mutton Sheep, 89 East Friesian Milk Sheep, 36 Rhön, 36 Pleven, 23 Tsigaja, 25 Black Razka and 86 Hungarian Merino X Pleven (F1) sheep were analysed by polyacrylamide gel electrophoresis under acid conditions and isoelectric focusing in ultrathin layer polyacrylamide gels with carrier ampholytes. Six different beta-lactoglobulin (beta-Lg) phenotypes (A, AB, B, AC, BC and C) were observed by both methods. The occurrence of three codominant alleles (beta-LgA, beta-LgB, beta-LgC) at an autosomal locus (beta-Lg) was supported by family and population data on genetic equilibrium. Differences in gene frequencies between the breeds were observed.

摘要

对来自189只美利奴兰羊、145只黑脸肉羊、89只东弗里生奶羊、36只罗恩羊、36只普列文羊、23只齐加亚羊、25只黑拉兹卡羊和86只匈牙利美利奴×普列文（F1）羊的乳样，在酸性条件下通过聚丙烯酰胺凝胶电泳以及在带有载体两性电解质的超薄层聚丙烯酰胺凝胶中进行等电聚焦分析。两种方法均观察到六种不同的β-乳球蛋白（β-Lg）表型（A、AB、B、AC、BC和C）。关于遗传平衡的家系和群体数据支持在常染色体位点（β-Lg）上存在三个共显性等位基因（β-LgA、β-LgB、β-LgC）。观察到各品种之间基因频率存在差异。