一种改进的绵羊血浆α1-蛋白酶抑制剂（PI1）变体分型方法。

An improved method for typing of plasma alpha 1-protease inhibitor (PI1) variants in sheep.

作者信息

Estomba A, Basaras M, Aguirre A, Vicario A, Jugo B, Mazon L I

机构信息

Department of Animal Biology and Genetics, Faculty of Sciences, University of the Basque Country, Bilbao, Spain.

出版信息

Anim Genet. 1993 Apr;24(2):133-4. doi: 10.1111/j.1365-2052.1993.tb00255.x.

DOI:10.1111/j.1365-2052.1993.tb00255.x

PMID:8328695

Abstract

Sheep plasma alpha 1-protease inhibitor (PI1) variants were typed by analysing neuraminidase treated samples by isoelectric focusing in ultrathin polyacrylamide gels followed by staining for trypsin inhibition. The 10 different PI1 phenotypes observed were shown to be controlled by four codominant alleles. The probability of sire exclusion provided by PI1 polymorphism was about 0.45 in both of the studied milk sheep breeds (Latxa and Karrantzar) of the Basque Country.

摘要

通过在超薄聚丙烯酰胺凝胶中进行等电聚焦分析经神经氨酸酶处理的样品，随后进行胰蛋白酶抑制染色，对绵羊血浆α1-蛋白酶抑制剂（PI1）变体进行分型。所观察到的10种不同的PI1表型显示由四个共显性等位基因控制。在巴斯克地区的两个研究奶羊品种（拉查羊和卡兰特萨尔羊）中，PI1多态性提供的父系排除概率约为0.45。