Department of Chemistry "G. Ciamician", Microchemistry and Microscopy Art Diagnostic Laboratory (M2ADL), University of Bologna, Ravenna Campus, Via Guaccimanni 42, 48100, Ravenna, Italy.
Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
Top Curr Chem (Cham). 2016 Jun;374(3):32. doi: 10.1007/s41061-016-0033-3. Epub 2016 May 10.
The present review is aimed at reporting on the most advanced and recent applications of immunochemical imaging techniques for the localization of proteins within complex and multilayered paint stratigraphies. Indeed, a paint sample is usually constituted by the superimposition of different layers whose characterization is fundamental in the evaluation of the state of conservation and for addressing proper restoration interventions. Immunochemical methods, which are based on the high selectivity of antigen-antibody reactions, were proposed some years ago in the field of cultural heritage. In addition to enzyme-linked immunosorbent assays for protein identification, immunochemical imaging methods have also been explored in the last decades, thanks to the possibility to localize the target analytes, thus increasing the amount of information obtained and thereby reducing the number of samples and/or analyses needed for a comprehensive characterization of the sample. In this review, chemiluminescent, spectroscopic and electrochemical imaging detection methods are discussed to illustrate potentialities and limits of advanced immunochemical imaging systems for the analysis of paint cross-sections.
本综述旨在报告免疫化学成像技术在复杂多层涂料层中蛋白质定位的最新应用。实际上，涂料样品通常由不同层的叠加组成，其特征对于评估保存状态和进行适当的修复干预至关重要。免疫化学方法基于抗原-抗体反应的高选择性，几年前就在文化遗产领域提出。除了用于蛋白质鉴定的酶联免疫吸附测定法外，免疫化学成像方法也在过去几十年中得到了探索，这要归功于能够定位目标分析物，从而增加了获得的信息量，从而减少了对样品进行全面表征所需的样品和/或分析的数量。在本综述中，讨论了化学发光、光谱和电化学成像检测方法，以说明先进的免疫化学成像系统在分析涂料横截面方面的潜力和限制。
