AP-HP, Hôpital Necker-Enfants Malades, Service de pneumologie et d'allergologie pédiatriques, Paris, France; Université Paris Descartes, Paris, France; Laboratory of Immunoregulation and Immunopathology, INEM (Institut Necker-Enfants Malades), CNRS UMR8253, and INSERM UMR1151, Paris, France.
AP-HP, Hôpital Necker-Enfants Malades, Service de pneumologie et d'allergologie pédiatriques, Paris, France.
Clin Immunol. 2018 Mar;188:7-11. doi: 10.1016/j.clim.2017.11.009. Epub 2017 Nov 28.
IL-17 and mucosal-associated invariant T (MAIT) cells have been involved in asthma pathogenesis. However, IL-17-producing MAIT cells (MAIT-17) were not evidenced. We aimed to determine whether circulating MAIT-17 were detectable in children with asthma, and whether they correlated with asthma symptoms or lung function. Children from the SPASM cohort of preschoolers with severe wheeze were reassessed for asthma at school age, and categorized as exacerbators (1 or more severe exacerbations in the previous 12months) or non-exacerbators. Nineteen children (10.9years) were included (9 non-exacerbators, 10 exacerbators). Circulating MAIT-17 were detected by flow cytometry. Their frequency was higher in exacerbators than in non-exacerbators (1.9 [1.01-3.55] vs 0.58 [0.46-1.15], p<0.01). MAIT-17 correlated with the number of severe exacerbations (r=0.68, p<0.001), and correlated negatively with the ACT score (r=-0.55, p=0.01). In summary, MAIT-17 are present in children with asthma and associated with asthma symptoms.
IL-17 和黏膜相关不变 T (MAIT) 细胞已被涉及到哮喘发病机制中。然而，尚未有证据表明存在产生 IL-17 的 MAIT 细胞（MAIT-17）。我们旨在确定哮喘患儿的循环 MAIT-17 是否可检测，以及它们是否与哮喘症状或肺功能相关。来自学龄前儿童严重喘息 SPASM 队列的儿童在学龄期重新评估哮喘，并分为加重者（过去 12 个月内有 1 次或多次严重加重）或非加重者。纳入了 19 名儿童（10.9 岁）（9 名非加重者，10 名加重者）。通过流式细胞术检测循环 MAIT-17。加重者的频率高于非加重者（1.9 [1.01-3.55] 比 0.58 [0.46-1.15]，p<0.01）。MAIT-17 与严重加重次数相关（r=0.68，p<0.001），与 ACT 评分呈负相关（r=-0.55，p=0.01）。总之，MAIT-17 存在于哮喘患儿中，与哮喘症状相关。
