海洋细菌 Cobetia litoralis KMM 3880(T) 的 O-多糖硫酸酯的结构与抗癌活性。
Structure and anticancer activity of sulfated O-polysaccharide from marine bacterium Cobetia litoralis KMM 3880(T).
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.
出版信息
Carbohydr Polym. 2016 Dec 10;154:55-61. doi: 10.1016/j.carbpol.2016.08.036. Epub 2016 Aug 12.
We presented the structure of the polysaccharide moiety and anticancer activity in vitro of the sulfated lipopolysaccharide isolated from the marine bacterium Cobetia litoralis KMM 3880(T). The structure of O-polysaccharide was investigated by chemical methods along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was built up of branched trisaccharide repeating units consist of D-glucose (D-Glcр), D-mannose (D-Manр) and sulfated 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo5S): →7-β-Kdoр4Ac5S-(2→4)-[β-d-Glcp-(1→2)-]-β-d-Manр6Ac-1→. We demonstrated that the lipopolysaccharide and О-deacetylated O-polysaccharide from Cobetia litoralis KMM 3880(T) inhibited a colony formation of human melanoma SK-MEL-28 and colorectal carcinoma HTC-116 cells.
我们介绍了从海洋细菌 Cobetia litoralis KMM 3880(T) 中分离出的硫酸化脂多糖的多糖部分结构和体外抗癌活性。通过化学方法以及（1）H 和（13）C NMR 光谱研究了 O-多糖的结构。O-多糖由支链三糖重复单元组成，由 D-葡萄糖（D-Glcр）、D-甘露糖（D-Manр）和硫酸化 3-去氧-D-甘露-2-辛酮酸（Kdo5S）组成：→7-β-Kdoр4Ac5S-（2→4）-[β-d-Glcp-（1→2）]-β-d-Manр6Ac-1→。我们证明 Cobetia litoralis KMM 3880(T) 的脂多糖和 O-去乙酰化 O-多糖抑制了人黑色素瘤 SK-MEL-28 和结直肠癌细胞 HTC-116 的集落形成。
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