Babes Liane, Kubes Paul
Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2016;1458:203-15. doi: 10.1007/978-1-4939-3801-8_15.
Intravital microscopy has evolved into an invaluable technique to study the complexity of tumors by visualizing individual cells in live organisms. Here, we describe a method for employing intravital spinning disk confocal microscopy to picture high-resolution tumor-stroma interactions in real time. We depict in detail the surgical procedures to image various tumor microenvironments and different cellular components in the liver.
活体显微镜检查已发展成为一种通过可视化活生物体中的单个细胞来研究肿瘤复杂性的宝贵技术。在此，我们描述一种利用活体旋转盘共聚焦显微镜实时描绘高分辨率肿瘤-基质相互作用的方法。我们详细描述了对肝脏中各种肿瘤微环境和不同细胞成分进行成像的手术过程。
