碘化丙啶嵌入溶液中微型RNA和DNA双链体插入位点处的糖环构象。
Sugar pucker geometries at the intercalation site of propidium diiodide into miniature RNA and DNA duplexes in solution.
作者信息
Patel D J, Shen C
出版信息
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2553-7. doi: 10.1073/pnas.75.6.2553.
Abstract
We have evaluated the sugar pucker geometry at the intercalation site of propidium diiodide into the self-complementary dinucleoside monophosphate duplexes cytidylylguanosine and deoxycytidylyldeoxyguanosine as a function of the nucleotide/drug ratio in aqueous solution. Our solution results support the observation by Sobell and coworkers [Sobell, H.M., Tsai, C.C., Jain, S.C. & Gilbert, S.G. (1977) J. Mol. Biol. 114, 333--365] of a C3' endo (3'-5')C2' endo sugar pucker geometry in the 2:2 intercalation complex of ethidium bromide into the iodocytidylylguanosine duplex in the crystalline state. We demonstrate further that the mixed sugar pucker observed for the intercalation of propidium diiodide into the miniature RNA duplex in solution persists in the intercalative complex of this trypanocidal drug into the corresponding miniature DNA duplex in solution.
摘要
我们评估了碘化丙啶嵌入自互补二核苷酸单磷酸双链体胞苷酰鸟苷和脱氧胞苷酰脱氧鸟苷的嵌入位点处的糖折叠几何结构，作为水溶液中核苷酸/药物比例的函数。我们的溶液结果支持了索贝尔及其同事[索贝尔，H.M.，蔡，C.C.，贾因，S.C.和吉尔伯特，S.G.（1977年）《分子生物学杂志》114，333 - 365]在结晶状态下溴化乙锭嵌入碘胞苷酰鸟苷双链体的2:2嵌入复合物中观察到的C3'内型（3'-5'）C2'内型糖折叠几何结构。我们进一步证明，在溶液中观察到的碘化丙啶嵌入微型RNA双链体时的混合糖折叠，在这种杀锥虫药物嵌入溶液中相应微型DNA双链体的嵌入复合物中依然存在。
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