单叠氮溴化乙锭的光谱性质：一种用于核酸的荧光光亲和标记物。
Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids.
作者信息
Bolton P H, Kearns D R
出版信息
Nucleic Acids Res. 1978 Dec;5(12):4891-903. doi: 10.1093/nar/5.12.4891.
Abstract
The non-covalent binding of ethidium monoazide to nucleic acids is entirely analogous to that of ethidium (binding constant approximately 2-3 X 10(5) M). The ethidium monoazide can be photochemically covalently linked to nucleic acids in high yield, up to 75%, by long wavelength light. The fluorescence of ethidium monoazide and ethidium crosslinked to nucleic acids show the same environmental sensitivity as does the fluorescence of ethidium. These properties of ethidium monoazide indicate its use as a fluorescent photoaffinity label for nucleic acids. Ethidium diazide can be photochemically linked to nucleic acids but appears to have properties substantially different from those of ethidium.
摘要
单叠氮溴化乙锭与核酸的非共价结合与溴化乙锭的结合完全类似（结合常数约为2 - 3×10⁵ M）。通过长波长光，单叠氮溴化乙锭能够以高达75%的高产率光化学共价连接到核酸上。与核酸交联的单叠氮溴化乙锭和溴化乙锭的荧光表现出与溴化乙锭荧光相同的环境敏感性。单叠氮溴化乙锭的这些特性表明它可作为核酸的荧光光亲和标记物。二叠氮溴化乙锭可通过光化学连接到核酸上，但似乎具有与溴化乙锭截然不同的性质。
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X-ray crystallographic visualization of drug-nucleic acid intercalative binding: structure of an ethidium-dinucleoside monophosphate crystalline complex, Ethidium: 5-iodouridylyl (3'-5') adenosine.药物与核酸插入结合的X射线晶体学可视化：溴化乙锭-二核苷单磷酸晶体复合物的结构，溴化乙锭：5-碘尿苷酰基（3'-5'）腺苷。
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