Reynolds J N, Carlen P L
Playfair Neuroscience Unit, Toronto Western Hospital, Addiction Research Foundation, Toronto, Ont., Canada.
Can J Physiol Pharmacol. 1989 Apr;67(4):359-62. doi: 10.1139/y89-057.
The effects of midazolam (3 nM) perfusion on the membrane and synaptic properties of dentate gyrus granule neurons were examined in hippocampal slices obtained from young adult (4-6 months) and old (24-26 months) Fischer 344 rats. In young neurons, midazolam perfusion resulted in a hyperpolarization of the resting membrane potential with no apparent change in the input resistance. Midazolam perfusion also produced a significant increase in the amplitude of the post-spike train afterhyperpolarization (AHP). In neurons obtained from old animals, midazolam perfusion also produced a hyperpolarization of the resting membrane potential but did not significantly change the AHP. These effects may result from altered calcium homeostasis in neurons of the aged brain, and suggest that at least some of the direct actions of benzodiazepines on mammalian central neurons are altered during aging.
在从年轻成年（4 - 6个月）和老年（24 - 26个月）的Fischer 344大鼠获取的海马切片中，研究了咪达唑仑（3 nM）灌注对齿状回颗粒神经元的膜特性和突触特性的影响。在年轻神经元中，咪达唑仑灌注导致静息膜电位超极化，输入电阻无明显变化。咪达唑仑灌注还使锋电位后超极化（AHP）的幅度显著增加。在老年动物的神经元中，咪达唑仑灌注也使静息膜电位超极化，但对AHP没有显著影响。这些效应可能是由于老年大脑神经元中钙稳态的改变所致，表明在衰老过程中，苯二氮䓬类药物对哺乳动物中枢神经元的至少一些直接作用发生了改变。
