远程缺血预处理减轻体外循环期间的氧化应激。
Remote Ischemic Preconditioning Attenuates Oxidative Stress during Cardiopulmonary Bypass.
作者信息
Arvola Oiva, Haapanen Henri, Herajärvi Johanna, Anttila Tuomas, Puistola Ulla, Karihtala Peeter, Anttila Vesa, Juvonen Tatu
机构信息
Research Unit of Surgery, Anesthesia and Intensive Care, University of Oulu and MRC Oulu, Finland.
Department of Obstetrics and Gynaecology, MRC Oulu, Oulu University Hospital and University of Oulu, Finland.
出版信息
Heart Surg Forum. 2016 Aug 23;19(4):E192-7. doi: 10.1532/hsf.1590.
BACKGROUND
Deep hypothermic circulatory arrest (DHCA) is used to overcome the threat of cerebral ischemia during complex surgical operations of the heart and the aortic arch. Remote ischemic preconditioning (RIPC) has been shown to mitigate neurological damage.
METHODS
We analyzed blood samples in a consecutive series of 52 piglets that underwent a 60-min period of DHCA with RIPC (the RIPC group) or without (the control group), to reveal whether the protective effect to oxidative stress could be seen by measuring serum 8-hydroxydeoxyguanosine (8-OHdG). The piglets were cannulated and cooled to 18°C using a heart-lung machine, for the DHCA. The piglets were then rewarmed to normothermic temperature. Blood sampling was taken at baseline, after 30 minutes of cooling, 2 hours postoperatively, and 8 hours postoperatively, and analyzed. 8-hydroxydeoxyguanosine (8-OHdG) from blood samples was analyzed by using Enzyme Linked Immunosorbent Assay (ELISA).
RESULTS
The serum 8-OHdG concentration was lower in the RIPC group after the cooling phase, 1.84 (1.44-2.17) ng/mL, and at 8 hours after HCA 1.48 (1.39-1.69) ng/mL, when compared with the control group, where the values were 2.14 (1.81-2.56) and 1.84 (1.62-2.44) ng/mL, respectively (P = .025) and (P = .004).
CONCLUSION
Remote ischemic preconditioning lowers oxidative stress during cardiopulmonary bypass.
背景
在心脏和主动脉弓的复杂外科手术中，深低温停循环（DHCA）用于克服脑缺血的威胁。远程缺血预处理（RIPC）已被证明可减轻神经损伤。
方法
我们分析了连续52只仔猪的血样，这些仔猪接受了60分钟的DHCA，其中一组采用RIPC（RIPC组），另一组未采用（对照组），通过测量血清8-羟基脱氧鸟苷（8-OHdG）来揭示对氧化应激的保护作用是否可见。仔猪插管后使用心肺机冷却至18°C进行DHCA。然后将仔猪复温至正常体温。在基线、冷却30分钟后、术后2小时和术后8小时采集血样并进行分析。采用酶联免疫吸附测定（ELISA）分析血样中的8-羟基脱氧鸟苷（8-OHdG）。
结果
与对照组相比，RIPC组在冷却阶段后血清8-OHdG浓度较低，为1.84（1.44-2.17）ng/mL，在心脏停搏后8小时为1.48（1.39-1.69）ng/mL，而对照组的值分别为2.14（1.81-2.56）和1.84（1.62-2.44）ng/mL（P = 0. 025）和（P = .004）。
结论
远程缺血预处理可降低体外循环期间的氧化应激。
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