Mieszkowski Jan, Stankiewicz Błaz Ej, Kochanowicz Andrzej, Niespodziński Bartłomiej, Borkowska Andz Elika, Sikorska Katarzyna, Daniłowicz-Szymanowicz Ludmiła, Brzezińska Paulina, Antosiewicz Jędrzej
Department of Gymnastics and Dance, Gdansk University of Physical Education and Sport, Gdańsk, Poland.
Faculty of Physical Education and Sport, Charles University, Prague, Czechia.
Front Physiol. 2021 Sep 6;12:731889. doi: 10.3389/fphys.2021.731889. eCollection 2021.
Clinical studies continue to provide evidence of organ protection by remote ischemic preconditioning (RIPC). However, there is lack of insight into impact of RIPC on exercise-induce changes in human organs' function. We here aimed to elucidate the effects of 10-day RIPC training on marathon-induced changes in the levels of serum markers of oxidative stress, and liver and heart damage. The study involved 18 male amateur runners taking part in a marathon. RIPC training was performed in the course of four cycles, by inflating and deflating a blood pressure cuff at 5-min intervals (RIPC group, =10); the control group underwent sham training (=8). The effects of RIPC on levels of oxidative stress, and liver and heart damage markers were investigated at rest after 10 consecutive days of training and after the marathon run. The 10-day RIPC training decreased the serum resting levels of C-reactive protein (CRP), alanine transaminase (ALT), γ-glutamyl transpeptidase (GGT), and malondialdehyde (MDA). After the marathon run, creatinine kinase MB (CK-MB), lactate dehydrogenase (LDH), cardiac troponin level (cTn), aspartate aminotransferase (AST), alkaline phosphatase (ALP), ALT, total bilirubin (BIL-T), and MDA levels were increased and arterial ketone body ratio (AKBR) levels were decreased in all participants. The changes were significantly diminished in the RIPC group compared with the control group. The GGT activity remained constant in the RIPC group but significantly increased in the control group after the marathon run. In conclusion, the study provides evidence for a protective effect of RIPC against liver and heart damage induced by strenuous exercise, such as the marathon.
临床研究不断提供远程缺血预处理（RIPC）对器官具有保护作用的证据。然而，对于RIPC对运动引起的人体器官功能变化的影响，目前仍缺乏深入了解。我们的目的是阐明为期10天的RIPC训练对马拉松运动引起的氧化应激血清标志物水平以及肝脏和心脏损伤的影响。该研究纳入了18名参加马拉松的男性业余跑步者。RIPC训练分四个周期进行，通过每隔5分钟给血压袖带充气和放气来实施（RIPC组，n = 10）；对照组进行假训练（n = 8）。在连续训练10天后的休息状态以及马拉松赛后，研究了RIPC对氧化应激水平以及肝脏和心脏损伤标志物的影响。为期10天的RIPC训练降低了血清中C反应蛋白（CRP）、丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（GGT）和丙二醛（MDA）的静息水平。马拉松赛后，所有参与者的肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、心肌肌钙蛋白水平（cTn）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、ALT、总胆红素（BIL-T）和MDA水平均升高，而动脉酮体比率（AKBR）水平降低。与对照组相比，RIPC组的这些变化明显减小。马拉松赛后，RIPC组的GGT活性保持不变，而对照组的GGT活性显著增加。总之，该研究为RIPC对剧烈运动（如马拉松）引起的肝脏和心脏损伤具有保护作用提供了证据。
