Beutler E, Guinto E, Kuhl W, Matsumoto F
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2825-8. doi: 10.1073/pnas.75.6.2825.
The question of whether separate "membrane" and "soluble" pools of ATP exist in erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived ("membrane") ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived ("soluble")ATP is not labeled under these circumstances. The specific activity of the gamma-phosphate of "soluble" ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP gama-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.
红细胞中是否存在独立的“膜结合”和“可溶性”ATP池这一问题已得到研究。通过与无机[32P]磷酸盐短期孵育，标记磷酸甘油酸激酶（EC 2.7.2.3）产生的（“膜结合”）ATP。在这些情况下，丙酮酸激酶（EC 2.7.1.40）产生的（“可溶性”）ATP未被标记。然后通过添加2-脱氧葡萄糖并测量形成的2-脱氧葡萄糖-6-[32P]磷酸的比活性，来评估“可溶性”ATP的γ-磷酸的比活性。该比活性与红细胞ATPγ-磷酸的总体比活性基本相同，表明红细胞中不存在磷酸甘油酸激酶产生的和丙酮酸激酶产生的ATP的功能性池。
