Ismail Nor Asiah, Rafii M Y, Mahmud T M M, Hanafi M M, Miah Gous
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Malaysia Agricultural Research and Development Institute, Serdang, Selangor, Malaysia.
Mol Biol Rep. 2016 Dec;43(12):1347-1358. doi: 10.1007/s11033-016-4070-3. Epub 2016 Sep 1.
Ginger is an economically important and valuable plant around the world. Ginger is used as a food, spice, condiment, medicine and ornament. There is available information on biochemical aspects of ginger, but few studies have been reported on its molecular aspects. The main objective of this review is to accumulate the available molecular marker information and its application in diverse ginger studies. This review article was prepared by combing material from published articles and our own research. Molecular markers allow the identification and characterization of plant genotypes through direct access to hereditary material. In crop species, molecular markers are applied in different aspects and are useful in breeding programs. In ginger, molecular markers are commonly used to identify genetic variation and classify the relatedness among varieties, accessions, and species. Consequently, it provides important input in determining resourceful management strategies for ginger improvement programs. Alternatively, a molecular marker could function as a harmonizing tool for documenting species. This review highlights the application of molecular markers (isozyme, RAPD, AFLP, SSR, ISSR and others such as RFLP, SCAR, NBS and SNP) in genetic diversity studies of ginger species. Some insights on the advantages of the markers are discussed. The detection of genetic variation among promising cultivars of ginger has significance for ginger improvement programs. This update of recent literature will help researchers and students select the appropriate molecular markers for ginger-related research.
生姜是一种在全球具有重要经济价值的植物。生姜被用作食物、香料、调味品、药物和装饰品。关于生姜的生化方面已有相关信息,但关于其分子方面的研究报道较少。本综述的主要目的是积累现有的分子标记信息及其在各种生姜研究中的应用。这篇综述文章是通过梳理已发表文章的材料和我们自己的研究成果撰写而成的。分子标记能够通过直接获取遗传物质来鉴定和表征植物基因型。在农作物物种中,分子标记应用于不同方面,对育种计划很有用。在生姜中,分子标记通常用于识别遗传变异并对品种、种质和物种之间的亲缘关系进行分类。因此,它为确定生姜改良计划的有效管理策略提供了重要依据。此外,分子标记还可以作为记录物种的协调工具。本综述重点介绍了分子标记(同工酶、随机扩增多态性DNA、扩增片段长度多态性、简单序列重复、简单序列重复区间扩增以及其他如限制性片段长度多态性、序列特征化扩增区域、核苷酸结合位点和单核苷酸多态性等)在生姜物种遗传多样性研究中的应用。还讨论了这些标记的一些优势。检测生姜优良品种之间的遗传变异对生姜改良计划具有重要意义。这篇近期文献综述将有助于研究人员和学生为生姜相关研究选择合适的分子标记。