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用于侵袭控制的大脑转录组学资源。

Transcriptomic Resources in the Brain of for Invasion Control.

作者信息

Wang Miao, Li Hanyu, Zheng Huoqing, Zhao Liuwei, Xue Xiaofeng, Wu Liming

机构信息

Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Insects. 2020 Feb 3;11(2):101. doi: 10.3390/insects11020101.

Abstract

The invasion of presents a great threat to the agriculture economy, the ecological environment, and human health. An effective strategy for this hornet control is urgently required, but the limited genome information of restricts the application of molecular-genomic tools for targeted hornet management. Therefore, we conducted large-scale transcriptome profiling of the hornet brain to obtain functional target genes and molecular markers. Using an Illumina HiSeq platform, more than 41 million clean reads were obtained and assembled into 182,087 meaningful unigenes. A total of 56,400 unigenes were annotated against publicly available protein sequence databases and a set of reliable Simple Sequence Repeats (SSRs) and Single Nucleotide Polymorphisms (SNP) markers were developed. The homologous genes encoding crucial behavior regulation factors, odorant binding proteins (OBPs), and vitellogenin, were also identified from highly expressed transcripts. This study provides abundant molecular targets and markers for invasive hornet control and further promotes the genetic and molecular study of .

摘要

黄蜂的入侵对农业经济、生态环境和人类健康构成了巨大威胁。迫切需要一种有效的黄蜂控制策略,但黄蜂有限的基因组信息限制了分子基因组工具在靶向黄蜂管理中的应用。因此,我们对黄蜂大脑进行了大规模转录组分析,以获得功能靶基因和分子标记。使用Illumina HiSeq平台,获得了超过4100万个干净读段,并组装成182087个有意义的单基因。共有56400个单基因在公开可用的蛋白质序列数据库中进行了注释,并开发了一组可靠的简单序列重复(SSR)和单核苷酸多态性(SNP)标记。还从高表达转录本中鉴定出编码关键行为调节因子、气味结合蛋白(OBP)和卵黄蛋白原的同源基因。本研究为入侵黄蜂的控制提供了丰富的分子靶点和标记,并进一步推动了黄蜂的遗传和分子研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d9/7074412/c9e8f6526ccd/insects-11-00101-g001.jpg

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