Newman E S, Moskie L A, Duggal R N, Goldenberg D M, Hansen H J
Immunomedics Inc., Newark, NJ 07103.
Clin Chem. 1989 Aug;35(8):1743-6.
We have previously reported that human anti-mouse IgG antibody (HAMA) can cause false-positive and false-negative results in "sandwich"-type monoclonal antibody (MAb) assays. To eliminate HAMA interference in "sandwich"-type MAb assays, we investigated the use of MAb on solid-phase, vinylidene fluoride floccules, which we have previously used as a solid-phase second antibody for RIA. The simple procedure effectively removes greater than 95% of HAMA from the most positive serum we have obtained from patients hyperimmunized to murine MAb, and it allows for accurate quantification of carcinoembryonic antigen. The solid-phase complex, added to blood, effectively removes HAMA and (or) "HAMA-type" heterophilic antibody from the sera or plasma.
我们之前曾报道，人抗小鼠IgG抗体（HAMA）可在“夹心”型单克隆抗体（MAb）检测中导致假阳性和假阴性结果。为消除HAMA对“夹心”型MAb检测的干扰，我们研究了将MAb用于固相偏二氟乙烯絮状物，我们之前曾将其用作放射免疫分析的固相二抗。该简单程序能有效从我们从超免疫小鼠MAb的患者获得的阳性血清中去除超过95%的HAMA，并且它允许对癌胚抗原进行准确定量。添加到血液中的固相复合物可有效从血清或血浆中去除HAMA和（或）“HAMA型”嗜异性抗体。
