De Leacy E A, Brown N N, Clague A E
Department of Pathology, Royal Brisbane Hospital, Australia.
Clin Chem. 1989 Aug;35(8):1772-4.
A patient who ingested a methanol/nitromethane mixture (model airplane fuel) showed an apparent plasma creatinine concentration of 8.0 mmol/L by the Jaffé reaction when the actual creatinine concentration, as measured by a specific enzymatic method, was 0.09 mmol/L. This effect was due to nitromethane in the plasma. Interference by nitromethane with the determination of creatinine by reaction with alkaline picrate (the Jaffé reaction) has not previously been reported. When nitromethane was added to plasma, the apparent creatinine measured was linearly related to the amount of nitromethane added. Comparison of spectral changes occurring during creatinine/picrate and nitromethane/picrate reactions show substantial similarity, suggesting a similar structure for the products. Although the findings are of interest for both their toxicological and analytical implications, the main interest lies with their suggestion of a model system for future investigation of the Jaffé reaction.
一名摄入甲醇/硝基甲烷混合物（模型飞机燃料）的患者，通过杰氏反应测得的血浆肌酐浓度明显为8.0 mmol/L，而用特定酶法测得的实际肌酐浓度为0.09 mmol/L。这种效应是由血浆中的硝基甲烷引起的。此前尚未报道过硝基甲烷对通过与碱性苦味酸盐反应（杰氏反应）测定肌酐的干扰。当向血浆中添加硝基甲烷时，测得的表观肌酐与添加的硝基甲烷量呈线性相关。比较肌酐/苦味酸盐和硝基甲烷/苦味酸盐反应过程中发生的光谱变化，发现有很大的相似性，这表明产物的结构相似。尽管这些发现因其毒理学和分析意义而备受关注，但主要兴趣在于它们为未来研究杰氏反应提供了一个模型系统的建议。
