一种使用非放射性同位素测量红细胞寿命的新方法。
A new approach for measuring the erythrocyte life span with a nonradioisotope.
作者信息
Dever M, Smith J E, Hausler D W
机构信息
Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
出版信息
Clin Chim Acta. 1989 May 31;181(3):337-42. doi: 10.1016/0009-8981(89)90239-8.
The possibility of substituting a nonradioactive isotope of chromium for 51Cr was studied. Blood, from five dogs with varying erythrocyte life spans, was labeled with 50Cr and 51Cr and transfused autologously. Erythrocyte-bound 50Cr was determined by inductively coupled argon plasma mass spectrometry and 51Cr by measuring radioactivity. Erythrocyte life span determination derived from the two isotopes did not differ significantly. Our results suggest that 50Cr can provide a nonradioactive alternative to 51Cr for labeling erythrocytes for life span studies.
研究了用非放射性铬同位素替代51Cr的可能性。采集了五只红细胞寿命不同的狗的血液，分别用50Cr和51Cr进行标记，然后进行自体输血。通过电感耦合氩等离子体质谱法测定红细胞结合的50Cr，通过测量放射性来测定51Cr。由这两种同位素得出的红细胞寿命测定结果没有显著差异。我们的结果表明，50Cr可以为红细胞寿命研究中标记红细胞提供一种替代51Cr的非放射性方法。
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