Wang Weishi, Cheng Min, Qiao Shupei, Wang Yuxiang, Li Hui, Wang Ning
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
Poult Sci. 2017 Jan 1;96(1):200-210. doi: 10.3382/ps/pew281. Epub 2016 Sep 1.
Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the gga-miR-21 mimic inhibited pre-adipocyte proliferation. In contrast, the gga-miR-21 inhibitor enhanced pre-adipocyte proliferation. The subsequent investigation identified Kruppel-like factor 5 (KLF5) mRNA as a target of gga-miR-21. The gga-miR-21 mimic inhibited KLF5 3UTR reporter activity and decreased endogenous KLF5 expression in primary pre-adipocytes. KLF5 knockdown using RNAi had a similar effect to that of the gga-miR-21 mimic on cell proliferation. The promoting effect of the gga-miR-21 inhibitor on pre-adipocyte proliferation was partially attenuated by KLF5 knockdown. Taken together, our results demonstrated that miR-21 inhibits chicken pre-adipocyte proliferation, at least in part, by targeting KLF5.
gga-miR-21在鸡前脂肪细胞中大量表达，但其作用尚不清楚。本研究探讨了gga-miR-21在鸡前脂肪细胞增殖中的作用。细胞增殖试验和增殖细胞核抗原（PCNA）的基因表达分析表明，gga-miR-21模拟物抑制前脂肪细胞增殖。相反，gga-miR-21抑制剂增强了前脂肪细胞增殖。随后的研究确定Kruppel样因子5（KLF5）mRNA是gga-miR-21的靶标。gga-miR-21模拟物抑制KLF5 3UTR报告基因活性，并降低原代前脂肪细胞中内源性KLF5的表达。使用RNAi敲低KLF5对细胞增殖的影响与gga-miR-21模拟物相似。gga-miR-21抑制剂对前脂肪细胞增殖的促进作用因KLF5敲低而部分减弱。综上所述，我们的结果表明miR-21至少部分通过靶向KLF5抑制鸡前脂肪细胞增殖。
