Department of Geriatric Cardiology, Chinese People’s Liberation Army General Hospital, Beijing, China
Food and Drug College, Anhui Science and Technology University, Fengyang, China
Turk J Med Sci. 2019 Jun 18;49(3):928-938. doi: 10.3906/sag-1710-173.
BACKGROUND/AIM: Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small noncoding microRNAs (miRNAs) have been considered critical modulators of the VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induced proinflammation and its atherogenic effect is still ambiguous.
The technique of qRT-PCR was used to examine the expression of miR-9 in VSMCs. The downstream signaling protein relative to miR-9 regulation, Krüppel-like factor 5, and some marker genes of contractile VSMCs were analyzed by western blotting and qRT-PCR. Luciferase reporter assay was used to detect the expression of KLF5, which is regulated by miR-9. To examine the function of a miR-9 inhibitor in VSMC proliferation and migration, VSMC proliferation and migration assays were performed.
Reduced transcriptional levels of miR-9 and expression of specific genes of contractile VSMCs were observed in the SMC cell line C-12511 treated with high glucose and SMCs, which were isolated from db/db mice. Moreover, the activity of KLF5 3′-UTR was dramatically reduced by a miR-9 mimic and increased by a miR-9 inhibitor. The proliferation and migration of SMCs were reduced by the miR-9 mimic.
miR-9 inhibits the proliferation and migration of SMC by targeting KLF5 in db/db mice.
背景/目的：糖尿病血管平滑肌细胞（VSMCs）的特征是增殖和迁移增加。小的非编码 microRNAs（miRNAs）被认为是环境刺激后 VSMC 表型转换的关键调节剂。然而，miRNA 在高糖诱导的促炎及其动脉粥样硬化作用仍然不清楚。
采用 qRT-PCR 技术检测 miR-9 在 VSMCs 中的表达。通过 Western blot 和 qRT-PCR 分析与 miR-9 调节相关的下游信号蛋白，Krüppel 样因子 5 和一些收缩型 VSMCs 的标记基因。利用荧光素酶报告实验检测受 miR-9 调节的 KLF5 的表达。为了研究 miR-9 抑制剂在 VSMC 增殖和迁移中的作用，进行了 VSMC 增殖和迁移实验。
在高糖处理的 C-12511 平滑肌细胞系和从 db/db 小鼠分离的 SMC 中，观察到 miR-9 的转录水平降低和收缩型 VSMCs 的特定基因表达降低。此外，miR-9 模拟物显著降低了 KLF5 3′-UTR 的活性，而 miR-9 抑制剂则增加了其活性。miR-9 模拟物降低了 SMC 的增殖和迁移。
miR-9 通过靶向 db/db 小鼠中的 KLF5 抑制 SMC 的增殖和迁移。
Zotero 插件
