Wu Zhaoming, Epasinghe Don Jeevanie, He Jinquan, Li Liwen, Green David W, Lee Min-Jung, Jung Han-Sung
Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, People's Republic of China.
Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangzhou Medical College, Guangzhou, Guangdong Province, People's Republic of China.
Cell Tissue Res. 2016 Dec;366(3):617-621. doi: 10.1007/s00441-016-2489-4. Epub 2016 Sep 3.
Vangl2, one of the core components of the planar cell polarity (PCP) pathway, has an important role in the regulation of morphogenesis in several tissues. Although the expression of Vangl2 has been detected in the developing tooth, its role in tooth morphogenesis is not known. In this study, we show that Vangl2 is expressed in the inner dental epithelium (IDE) and in the secondary enamel knots (SEKs) of bell stage tooth germs. Inhibition of Vangl2 expression by siRNA treatment in in vitro-cultured tooth germs resulted in retarded tooth germ growth with deregulated cell proliferation and apoptosis. After kidney transplantation of Vangl2 siRNA-treated tooth germs, teeth were observed to be small and malformed. We also show that Vangl2 is required to maintain the proper pattern of cell alignment in SEKs, which maybe important for the function of SEKs as signaling centers. These results suggest that Vangl2 plays an important role in the morphogenesis of teeth.
Vangl2是平面细胞极性（PCP）通路的核心组成部分之一，在多种组织的形态发生调控中发挥重要作用。虽然在发育中的牙齿中已检测到Vangl2的表达，但其在牙齿形态发生中的作用尚不清楚。在本研究中，我们发现Vangl2在钟状期牙胚的内釉上皮（IDE）和继发性釉结（SEK）中表达。在体外培养的牙胚中，通过siRNA处理抑制Vangl2表达会导致牙胚生长迟缓，细胞增殖和凋亡失调。将经Vangl2 siRNA处理的牙胚移植到肾脏后，观察到牙齿小且畸形。我们还表明，Vangl2是维持SEK中细胞正确排列模式所必需的，这可能对SEK作为信号中心的功能很重要。这些结果表明，Vangl2在牙齿形态发生中起重要作用。
