Sanz-Navarro Maria, Seidel Kerstin, Sun Zhao, Bertonnier-Brouty Ludivine, Amendt Brad A, Klein Ophir D, Michon Frederic
Helsinki Institute of Life Sciences, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland.
Orthodontics, Department of Oral and Maxillofacial Diseases, University of Helsinki, 00290 Helsinki, Finland.
Development. 2018 Jan 8;145(1):dev155929. doi: 10.1242/dev.155929.
In mice, the incisors grow throughout the animal's life, and this continuous renewal is driven by dental epithelial and mesenchymal stem cells. is a principal marker of the epithelial stem cells that reside in the mouse incisor stem cell niche, called the labial cervical loop, but relatively little is known about the role of the stem cell population. In this study, we show that conditional deletion of in the embryonic incisor epithelium leads to growth defects and impairment of ameloblast lineage commitment. Deletion of specifically in cells during incisor renewal revealed cellular plasticity that leads to the relatively rapid restoration of a -expressing cell population. Furthermore, we show that -expressing cells are a subpopulation of dental cells that also arise from cells during tooth formation. Finally, we show that the embryonic and adult populations are regulated by distinct signalling pathways, which is reflected in their distinct transcriptomic signatures. Together, our findings demonstrate that a stem cell population can be regenerated from cells, reinforcing its importance for incisor homeostasis.
在小鼠中，门齿在动物的整个生命过程中持续生长，这种持续更新由牙齿上皮和间充质干细胞驱动。是位于小鼠门齿干细胞龛（称为唇侧颈环）中的上皮干细胞的主要标志物，但对于该干细胞群体的作用了解相对较少。在本研究中，我们表明在胚胎门齿上皮中条件性缺失会导致生长缺陷和成釉细胞谱系定向的受损。在门齿更新过程中特异性地在细胞中缺失显示出细胞可塑性，这导致表达细胞群体相对快速地恢复。此外，我们表明表达细胞是牙齿细胞的一个亚群，在牙齿形成过程中也起源于细胞。最后，我们表明胚胎和成体群体受不同的信号通路调控，这在它们不同的转录组特征中得到体现。总之，我们的研究结果表明一个干细胞群体可以从细胞再生，强化了其对门齿稳态的重要性。
