植物乳杆菌1-3的滚环复制质粒pM411的特性分析
Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1-3.
作者信息
Ma Xvchuan, Li Jinhua, Xiong Yao, Zhai Zhengyuan, Ren Fazheng, Hao Yanling
机构信息
Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
The Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
出版信息
Curr Microbiol. 2016 Dec;73(6):820-826. doi: 10.1007/s00284-016-1124-7. Epub 2016 Sep 3.
A cryptic plasmid pM411 isolated from Lactobacillus plantarum 1-3 consisted of a 2303-bp circular molecule with a G + C content 32.96 %. Sequence analysis of pM411 revealed four putative open reading frames (ORFs). ORF1 shared 99 and 94 % similarities, respectively, with the Rep proteins of plasmids pLC2 and pYC2, which belong to the rolling-circle replication pMV158 family. A typical pMV158 family double-strand origin (dso) and a putative single-strand origin (sso) located upstream of the rep gene. Southern hybridization confirmed the presence of single-strand DNA (ssDNA) intermediates, suggesting that pM411 belongs to the RCR pMV158 family. Sequence homology analysis indicated that the sso belongs to the ssoW family. Furthermore, the relative copy number of pM411 was about 88 copies in each cell by real-time PCR.
从植物乳杆菌1-3中分离出的隐秘质粒pM411由一个2303 bp的环状分子组成，其G + C含量为32.96%。pM411的序列分析揭示了四个推定的开放阅读框（ORF）。ORF1分别与属于滚环复制pMV158家族的质粒pLC2和pYC2的Rep蛋白具有99%和94%的相似性。一个典型的pMV158家族双链起点（dso）和一个推定的单链起点（sso）位于rep基因的上游。Southern杂交证实了单链DNA（ssDNA）中间体的存在，表明pM411属于RCR pMV158家族。序列同源性分析表明，sso属于ssoW家族。此外，通过实时PCR检测，pM411在每个细胞中的相对拷贝数约为88个。
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