Department of Cognitive Neurobiology, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, A-1090, Vienna, Austria.
Department of Cognitive Neurobiology, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, A-1090, Vienna, Austria.
Neuron. 2016 Sep 21;91(6):1390-1401. doi: 10.1016/j.neuron.2016.08.010. Epub 2016 Sep 1.
Parvalbumin-expressing basket cells tightly control cortical networks and fire remarkably stereotyped during network oscillations and simple behaviors. How can these cells support multifaceted situations with different behavioral options and complex temporal sequences? We recorded from identified parvalbumin-expressing basket cells in prefrontal cortex of freely moving rats performing a multidimensional delayed cue-matching-to-place task, juxtacellularly filled recorded neurons for unequivocal histological identification, and determined their activity during temporally structured task episodes, associative working-memory, and stimulus-guided choice behavior. We show that parvalbumin-expressing basket cells do not fire homogenously, but individual cells were recruited or inhibited during different task episodes. Firing of individual basket cells was correlated with amount of presynaptic VIP (vasoactive intestinal polypeptide)-expressing GABAergic input. Together with subsets of pyramidal neurons, activity of basket cells differentiated for sequential actions and stimulus-guided choice behavior. Thus, interneurons of the same cell type can be recruited into different neuronal ensembles with distinct firing patterns to support multi-layered cognitive computations.
表达钙结合蛋白 Parvalbumin 的篮状细胞紧密地控制着皮质网络的活动，并且在网络振荡和简单行为期间表现出非常刻板的放电模式。那么这些细胞如何在具有不同行为选项和复杂时间序列的多种情况下提供支持呢？我们在自由活动的大鼠的前额叶皮层中记录了被鉴定为表达钙结合蛋白 Parvalbumin 的篮状细胞，通过细胞内注射染料对记录神经元进行了明确的组织学鉴定，并确定了它们在时间结构任务事件、联想工作记忆和刺激引导的选择行为期间的活动。我们发现表达钙结合蛋白 Parvalbumin 的篮状细胞并非均匀放电，而是在不同的任务事件中募集或抑制个别细胞。单个篮状细胞的放电与前突触 VIP（血管活性肠肽）表达 GABA 能输入的量相关。与部分锥体神经元一起，篮状细胞的活动可以区分用于连续动作和刺激引导选择行为的神经元集合。因此，相同类型的中间神经元可以被募集到具有不同放电模式的不同神经元集合中，以支持多层次的认知计算。
