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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Margelin D, Pigier A M, Gardette J, Bertrand J, Vu-Dac N, Picard J

Laboratoire de Biochimie, INSERM U 181, Faculté de Médecine Saint-Antoine, Paris, France.

Int J Biochem. 1989;21(7):761-8. doi: 10.1016/0020-711x(89)90208-5.

Artery wall proteoglycans-lipoprotein lipase binding characteristics were studied using bovine milk 125I-labelled lipoprotein lipase (LPL) and chondroitin sulphate-dermatan sulphate proteoglycans (PGs) purified from pig aorta. 2. The binding process was studied either by a soluble assay (gel filtration) or by an immobilized proteoglycan assay (ELISA). 3. The binding process was reversible, saturable and occurred at a stoichiometry 1:1. 4. The binding process involved ionic interactions between the positively charged groups of LPL and the negatively charged groups of PG carbohydrate chains. 5. The complex PG-LPL may lead to the production of remnant lipoproteins and, thereby, contribute to cholesteryl ester accumulation in the arterial wall.

使用从猪主动脉中纯化的牛乳125I标记的脂蛋白脂肪酶（LPL）和硫酸软骨素-硫酸皮肤素蛋白聚糖（PGs）研究动脉壁蛋白聚糖-脂蛋白脂肪酶的结合特性。2. 通过可溶性测定法（凝胶过滤）或固定化蛋白聚糖测定法（ELISA）研究结合过程。3. 结合过程是可逆的、可饱和的，并且以1:1的化学计量比发生。4. 结合过程涉及LPL带正电基团与PG碳水化合物链带负电基团之间的离子相互作用。5. 复合物PG-LPL可能导致残余脂蛋白的产生，从而促进动脉壁中胆固醇酯的积累。

Margelin D, Pigier A M, Gardette J, Bertrand J, Vu-Dac N, Picard J

Laboratoire de Biochimie, INSERM U 181, Faculté de Médecine Saint-Antoine, Paris, France.

Int J Biochem. 1989;21(7):761-8. doi: 10.1016/0020-711x(89)90208-5.

Artery wall proteoglycans-lipoprotein lipase binding characteristics were studied using bovine milk 125I-labelled lipoprotein lipase (LPL) and chondroitin sulphate-dermatan sulphate proteoglycans (PGs) purified from pig aorta. 2. The binding process was studied either by a soluble assay (gel filtration) or by an immobilized proteoglycan assay (ELISA). 3. The binding process was reversible, saturable and occurred at a stoichiometry 1:1. 4. The binding process involved ionic interactions between the positively charged groups of LPL and the negatively charged groups of PG carbohydrate chains. 5. The complex PG-LPL may lead to the production of remnant lipoproteins and, thereby, contribute to cholesteryl ester accumulation in the arterial wall.

使用从猪主动脉中纯化的牛乳125I标记的脂蛋白脂肪酶（LPL）和硫酸软骨素-硫酸皮肤素蛋白聚糖（PGs）研究动脉壁蛋白聚糖-脂蛋白脂肪酶的结合特性。2. 通过可溶性测定法（凝胶过滤）或固定化蛋白聚糖测定法（ELISA）研究结合过程。3. 结合过程是可逆的、可饱和的，并且以1:1的化学计量比发生。4. 结合过程涉及LPL带正电基团与PG碳水化合物链带负电基团之间的离子相互作用。5. 复合物PG-LPL可能导致残余脂蛋白的产生，从而促进动脉壁中胆固醇酯的积累。