Vijayagopal P, Radhakrishnamurthy B, Srinivasan S R, Berenson G S
Lab Invest. 1980 Feb;42(2):190-6.
Chondroitin sulfate-dermatan sulfate proteoglycans (PG) were isolated from bovine aorta-intima by extraction with 4.0 M guanidinium chloride in the presence of protease inhibitors and purified through cetylpyridinium complexes. The PG were fractionated by CsCl isopyknic centrifugation into three fractions with different chemical composition. The anticoagulant activity of the PG fractions was studied by Stypven time, partial thromboplastin clotting time (PTT), and thrombin time assays. The three PG fractions delayed coagulation in the three assays. The PG fractions did not affect the ADP and collagen-induced platelet aggregation but inhibited the aggregation induced by 0.2 units per ml. of thrombin. The PG fractions released lipoprotein lipase in rabbits when injected, and the enzyme activity released by the major PG fraction was approximately 60 per cent of that of heparin. This PG fraction interacted with serum low density lipoproteins but not with high density lipoproteins. Certain biologic properties are probably due to the presence of dermatan sulfate in the PG fractions. These studies suggest important functional roles for PG in the arterial wall.
硫酸软骨素-硫酸皮肤素蛋白聚糖（PG）通过在蛋白酶抑制剂存在的情况下用4.0 M氯化胍提取从牛主动脉内膜中分离出来，并通过十六烷基吡啶络合物进行纯化。PG通过CsCl等密度离心法分为具有不同化学组成的三个部分。通过白陶土部分凝血活酶时间、部分凝血活酶凝血时间（PTT）和凝血酶时间测定研究了PG部分的抗凝活性。这三个PG部分在这三种测定中均延迟了凝血。PG部分不影响ADP和胶原诱导的血小板聚集，但抑制每毫升0.2单位凝血酶诱导的聚集。PG部分注射到兔子体内时会释放脂蛋白脂肪酶，主要PG部分释放的酶活性约为肝素的60%。该PG部分与血清低密度脂蛋白相互作用，但不与高密度脂蛋白相互作用。某些生物学特性可能归因于PG部分中硫酸皮肤素的存在。这些研究表明PG在动脉壁中具有重要的功能作用。
