Wójciak-Kosior Magdalena, Sowa Ireneusz, Pucek Kamila, Szymczak Grażyna, Kocjan Ryszard, Luchowski Piotr
a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.
b Botanical Garden of Maria Curie-Skłodowska University in Lublin , Lublin , Poland.
Pharm Biol. 2017 Dec;55(1):1-4. doi: 10.1080/13880209.2016.1225773. Epub 2016 Sep 4.
Viscum album L. (Loranthaceae) is a semi-parasitic plant used in pharmacy and medicine mostly for its hypotensive and anticancer activity. The effects may be related to the presence of triterpenic acids, such as betulinic (BA) and oleanolic (OA) acids.
In our investigations the content of triterpenic acids in V. album from different host trees depending on the season of harvest was determined.
V. album herb was dried and extracted with ethyl acetate using ultrasound energy. The reversed phase HPLC-PDA method was used for the analysis of triterpenic acids. The structure of the target components was confirmed by mass spectrometry with an electrospray ionization source.
Diversity in the content of both compounds was noted; however, OA was the dominant triterpenic acid and the amount thereof was ∼10 times higher than that of BA. The analysis of changes in the amount of triterpenic acids during the spring-winter period revealed the highest content of OA in summer (from 6.84 to 13.65 mg/g). In turn, in the other seasons of harvest, the content was in the range of 4.41-9.83, 6.41-9.56 and 5.59-12.16 mg/g for spring, autumn and winter, respectively. In most cases, a similar tendency was observed for BA.
In most cases, the highest amount of the investigated compounds was found in summer; thus, this period seems to be optimal for acquisition of plant material rich in triterpenic acids.
欧洲槲寄生（桑寄生科）是一种半寄生植物，主要因其降压和抗癌活性而用于制药。这些作用可能与三萜酸的存在有关，如桦木酸（BA）和齐墩果酸（OA）。
在我们的研究中，测定了不同寄主树上的欧洲槲寄生中三萜酸的含量，该含量取决于收获季节。
将欧洲槲寄生草本植物干燥，利用超声能量用乙酸乙酯萃取。采用反相高效液相色谱-光电二极管阵列检测法分析三萜酸。通过电喷雾电离源质谱法确认目标成分的结构。
注意到这两种化合物的含量存在差异；然而，OA是主要的三萜酸，其含量比BA高约10倍。对春冬季期间三萜酸含量变化的分析表明，夏季OA含量最高（从6.84至13.65毫克/克）。相应地，在其他收获季节，春季、秋季和冬季的含量分别在4.41 - 9.83、6.41 - 9.56和5.59 - 12.16毫克/克范围内。在大多数情况下，BA也观察到类似趋势。
在大多数情况下，所研究化合物的含量在夏季最高；因此，这个时期似乎是获取富含三萜酸植物材料的最佳时期。
