Ablashi D V, Lusso P, Hung C L, Salahuddin S Z, Josephs S F, Llana T, Kramarsky B, Biberfeld P, Markham P D, Gallo R C
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Dev Biol Stand. 1989;70:139-46.
In 1986, we reported the discovery and isolation of a novel human herpesvirus (HBLV) from AIDS and other lymphoproliferative disorders. Because HBLV is distinct from other members of the herpesvirus family and can infect B- and T-lymphocytes and other human cells (megakaryocytes and glioblastoma cells), we suggested human herpesvirus-6 (HHV-6) as the taxonomic designation for this virus. In cultures from patients' peripheral blood, the evidence of HBLV can be recognized from the appearance of short-lived giant cells (2-10%), which are large, refractile, and are often mono- and binucleated. As these cells degenerate, extracellular virus particles are found in the culture medium. HBLV can infect fresh mononuclear cells, established B- and T-lymphoblastoid cell lines, megakaryocytes and glioblastoma cell lines. HBLV infection can be detected by: a. morphological changes; b. indirect immunofluorescence assay, in situ hybridization, southern blot analysis, polymerase chain reaction amplification; and c. electron microscopy. Because of its wide cell tropism, HBLV DNA sequences have been detected in B-cell lymphomas and short term cultured cells from Sjogren's patients. Expression of HBLV RNA was also detected in sarcoidosis. The etiological role of HBLV in human tumors is unclear. While in vitro data may not necessarily apply to in vivo conditions, the infection of various cell lines from tumors and fresh mononuclear cells suggests HBLV involvement in a variety of diseases.
1986年，我们报告了从艾滋病患者及其他淋巴增生性疾病患者体内发现并分离出一种新型人类疱疹病毒（HBLV）。由于HBLV与疱疹病毒家族的其他成员不同，且能感染B淋巴细胞、T淋巴细胞及其他人类细胞（巨核细胞和成胶质细胞瘤细胞），我们建议将人类疱疹病毒6型（HHV-6）作为该病毒的分类名称。在患者外周血培养物中，可从短暂出现的巨大细胞（2%-10%）的形态识别出HBLV感染迹象，这些细胞体积大、有折光性，且常为单核或双核。随着这些细胞退化，可在培养基中发现细胞外病毒颗粒。HBLV可感染新鲜单核细胞、已建立的B淋巴细胞系和T淋巴细胞系、巨核细胞和成胶质细胞瘤细胞系。可通过以下方法检测HBLV感染：a. 形态学改变；b. 间接免疫荧光测定、原位杂交、Southern印迹分析、聚合酶链反应扩增；c. 电子显微镜检查。由于其广泛的细胞嗜性，在B细胞淋巴瘤及干燥综合征患者的短期培养细胞中已检测到HBLV DNA序列。在结节病中也检测到了HBLV RNA的表达。HBLV在人类肿瘤中的病因学作用尚不清楚。虽然体外数据不一定适用于体内情况，但HBLV对来自肿瘤的各种细胞系及新鲜单核细胞的感染表明其与多种疾病有关。
