Biberfeld P, Kramarsky B, Salahuddin S Z, Gallo R C
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
J Natl Cancer Inst. 1987 Nov;79(5):933-41.
A new DNA virus, designated "human B lymphotropic virus" or "human herpesvirus 6" (HBLV), has been isolated from the peripheral blood leukocytes of patients with various lymphoproliferative disorders, in some instances also associated with human immunodeficiency virus (HIV-1) infection. HBLV, propagated in vitro in human cord blood lymphocytes, was found to be ultrastructurally similar to members of the herpesvirus family. It is an enveloped virus with an icosahedral nucleocapsid made up of 162 capsomeres. Unenveloped nucleocapsids in the cytoplasm wee always coated with a tegument, a feature also described for human cytomegalovirus (HCMV). However, the nucleoprotein core of HBLV does not have the beaded appearance as that of HCMV, nor do HBLV-infected cells contain the skein-like structure. Immune electron microscopy demonstrated the presence of specific antibodies to viral envelope and internal antigens in sera of infected patients, indicating that this virus is a possible human pathogen. These and previously reported characteristics are consistent with the HBLV being a new and unique DNA virus morphologically belonging to the herpesvirus family.
一种新的DNA病毒，被命名为“人类嗜B淋巴细胞病毒”或“人类疱疹病毒6型”（HBLV），已从患有各种淋巴增生性疾病患者的外周血白细胞中分离出来，在某些情况下还与人类免疫缺陷病毒（HIV-1）感染有关。在人脐血淋巴细胞中进行体外培养的HBLV，在超微结构上被发现与疱疹病毒科的成员相似。它是一种包膜病毒，具有由162个壳粒组成的二十面体核衣壳。细胞质中的无包膜核衣壳总是被一层被膜包裹，这一特征在人类巨细胞病毒（HCMV）中也有描述。然而，HBLV的核蛋白核心没有HCMV那样的串珠状外观，感染HBLV的细胞也不包含丝状结构。免疫电子显微镜显示，感染患者血清中存在针对病毒包膜和内部抗原的特异性抗体，表明这种病毒可能是一种人类病原体。这些以及先前报道的特征与HBLV是一种形态上属于疱疹病毒科的新型独特DNA病毒相一致。
