Vural Murat, Cosan T Erhan, Uzuner Kubilay, Erkasap Nilufer, Cosan Didem, Bal Cengiz
Eskisehir Osmangazi University, School of Medicine, Department of Neurosurgery, Eskisehir, Turkey.
Turk Neurosurg. 2018;28(1):142-148. doi: 10.5137/1019-5149.JTN.18072-16.1.
To describe the relationship between the parenchymal pressure changes and the development of hydrocephalus in kaolininjected neonatal rats according to cerebral regions and time intervals of developing hydrocephalus.
Neonatal rats aged 2 to 3 days were examined in 5 groups as kaolin frontal "K-F", kaolin parietal "KP", saline frontal "SF-F", saline parietal "SF-P" and control "C", based on the injected material and injection sites. All injections were performed into the cortical subarachnoid space of the right frontal and right parietal regions. The fifth group was injection free. On the 3 < sup > rd < /sup > , 7 < sup > th < /sup > , 15 < sup > th < /sup > , 30 < sup > th < /sup > and 60 < sup > th < /sup > days after injection, parenchymal pressures (PP) of 5-7 rats from each group were measured from different regions.
We compared the control group with saline-injected and kaolin-injected groups and found statistically significant parenchymal pressure differences based on regional measurements. In the kaolin groups, the mean PP values were obviously higher than the saline-injected group. Within each kaolin-injected group, the pressure values were variable and inconsistent regarding the parenchymal regions.
Hydrocephalus cannot be totally explained with existent "bulk-flow" or "hydrodynamic" theories. Although our experimental design was planned to develop hydrocephalus according to the bulk flow theory, our results were more compatible with the hydrodynamic theory. The present comments on the occurrence and pathogenesis of hydrocephalus are still open to debate and may require further comprehensive studies.
根据高岭土注射新生大鼠脑积水发展的脑区和时间间隔,描述实质压力变化与脑积水发展之间的关系。
根据注射材料和注射部位,将2至3日龄的新生大鼠分为5组,即高岭土额叶组(“K-F”)、高岭土顶叶组(“K-P”)、生理盐水额叶组(“SF-F”)、生理盐水顶叶组(“SF-P”)和对照组(“C”)。所有注射均注入右侧额叶和右侧顶叶的皮质蛛网膜下腔。第五组不进行注射。在注射后的第3、7、15、30和60天,从每组中选取5至7只大鼠,测量不同脑区的实质压力(PP)。
我们将对照组与注射生理盐水组和注射高岭土组进行比较,发现基于区域测量的实质压力存在统计学上的显著差异。在高岭土组中,平均PP值明显高于注射生理盐水组。在每个注射高岭土的组内,不同实质区域的压力值变化不定且不一致。
现有的“体积流”或“流体动力学”理论无法完全解释脑积水的发生机制。尽管我们的实验设计是按照体积流理论来诱导脑积水,但我们的结果更符合流体动力学理论。目前关于脑积水发生和发病机制的观点仍存在争议,可能需要进一步的综合研究。