Navarrete Santos Alexander, Yan Junfeng, Lochmann Peter, Pfeil Heike, Petersen Michael, Simm Andreas, Treede Hendrik, Sievers Hans H, Mohamed Salah A
a Department of heart surgery , Martin Luther University , Halle-Wittenberg , Germany and.
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Arch Physiol Biochem. 2016 Dec;122(5):289-294. doi: 10.1080/13813455.2016.1211710. Epub 2016 Sep 5.
Dilatation of the ascending aorta is a common occurrence in patients with bicuspid aortic valve (BAV). The aim of the current study was to characterize collagen content in advanced glycation end products (AGEs) of dilated aortic tissue from two distinct areas, concave and convex aortic sites in patients with BAV and TAV. Collagen contents extracted from 100 mg tissue was isolated by enzymatic digestion using pepsin and the nondigested material was further digested using cyanogen bromide, insoluble collagen fraction (ICF) was extracted by hydrochloric acid hydrolysis. BAV tissue showed diminished fluorescence of the pepsin extracted fraction (PEF) compared with TAV tissue (12.4 ± 1.0% vs 32.9 ± 7.6%, p = 0.05). Patients with BAV had PEF of collagens significantly diminished in the dilated ascending aorta, especially in its convex portion, in course of aging and increment of dilated diameters. It is suggestible that BAV patients present more highly AGE-modified collagens in their ascending aorta.
升主动脉扩张在二叶式主动脉瓣（BAV）患者中很常见。本研究的目的是表征BAV和三叶式主动脉瓣（TAV）患者扩张主动脉组织两个不同区域（主动脉凹面和凸面）晚期糖基化终末产物（AGEs）中的胶原蛋白含量。从100mg组织中提取的胶原蛋白通过用胃蛋白酶进行酶消化来分离，未消化的物质再用溴化氰进一步消化，不溶性胶原蛋白部分（ICF）通过盐酸水解提取。与TAV组织相比，BAV组织的胃蛋白酶提取部分（PEF）荧光减弱（12.4±1.0%对32.9±7.6%，p = 0.05）。在衰老过程和扩张直径增加的过程中，BAV患者扩张升主动脉中的胶原蛋白PEF显著减少，尤其是在其凸面部分。提示BAV患者升主动脉中存在更多高度AGE修饰的胶原蛋白。
