Azorín I, Miñana M D, Felipo V, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Centro Asociado del Consejo Superior de Investigaciones Científicas, Spain.
Hepatology. 1989 Sep;10(3):311-4. doi: 10.1002/hep.1840100310.
Rats were fed a standard diet or the standard diet supplemented with ammonium acetate (20% w/w) for up to 100 days. The effect of the ingestion of the high-ammonium diet on some aspects of nitrogen metabolism in rats was studied. Ammonia levels in blood increased approximately 3-fold; in brain, liver and muscle the increases were 36, 34 and 50%, respectively. Urea levels in blood and urea excretion increased approximately 2-fold. There was no increase of carbamyl phosphate synthase. Liver glutamine synthase activity increased by 58% and glutamate dehydrogenase by 40%, whereas glutaminase was not affected. Glutamine content in brain was twice that of controls. This new animal model to study hyperammonemia offers several advantages over others: it is simpler, is bloodless, requires no animal manipulation and permits long-term studies.
给大鼠喂食标准饮食或添加醋酸铵（20% w/w）的标准饮食，持续100天。研究了摄入高铵饮食对大鼠氮代谢某些方面的影响。血液中的氨水平增加了约3倍；在脑、肝和肌肉中，增加幅度分别为36%、34%和50%。血液中的尿素水平和尿素排泄量增加了约2倍。氨甲酰磷酸合成酶没有增加。肝脏谷氨酰胺合成酶活性增加了58%，谷氨酸脱氢酶增加了40%，而谷氨酰胺酶未受影响。脑中谷氨酰胺含量是对照组的两倍。这种用于研究高氨血症的新动物模型比其他模型具有几个优点：它更简单、无出血、无需对动物进行操作且允许进行长期研究。
