Hussein Hassan A, Mohamed Ragab H, Hossam Mohamed, Wehrend Axel
Hassan A. Hussein, Theriogenology Department, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt, Email:
Tierarztl Prax Ausg G Grosstiere Nutztiere. 2016 Oct 12;44(5):287-294. doi: 10.15653/TPG-160214. Epub 2016 Sep 6.
The aim of this study was to monitor the ovarian response and conception rate following estrous synchronization using CIDR, Ovsynch and double prostaglandin F protocols in Egyptian buffalo heifers.
A total of 80 cyclic buffalo heifers were divided into four equal groups: CIDR (intravaginal progesterone releasing device, EAZI-BREED CIDR), Ovsynch (GnRH, PGF, GnRH injections), PGF (double PGF doses) and control. Timed artificial insemination (TAI) was performed in all heifers. All animals were examined using ultrasound and blood samples were collected for measurement of progesterone.
A new follicular wave occurred earlier in the Ovsynch and PGF groups than in the CIDR group (p < 0.05). The mean diameter of the ovulatory follicle was smaller in the CIDR group than in the Ovsynch and PGF groups (p < 0.05). The ovulation rate was 100% in the CIDR group, 75% in the Ovsynch group and 70% in the PGF group. In the control group a lower pregnancy rate (20%) was determined in than in the CIDR (35%), Ovsynch (40%) and PGF (35%) groups. Progesterone concentrations were numerically higher in pregnant heifers of the CIDR group but the difference was non-significantly compared to the Ovsynch, PGF and control groups (p > 0.05).
EAZI-BREED CIDR, Ovsynch-based TAI and PGF protocols were effective in synchronizing oestrus and resulted in nearly similar pregnancy rates in Egyptian buffalo heifers.
本研究旨在监测在埃及水牛小母牛中使用阴道孕酮释放装置（CIDR）、Ovsynch方案和双前列腺素F方案进行发情同步后的卵巢反应和受孕率。
总共80头处于发情周期的水牛小母牛被平均分为四组：CIDR组（阴道孕酮释放装置，EAZI-BREED CIDR）、Ovsynch组（促性腺激素释放激素、前列腺素F、促性腺激素释放激素注射）、PGF组（双剂量前列腺素F）和对照组。所有小母牛均进行定时人工授精（TAI）。对所有动物进行超声检查，并采集血样以测定孕酮。
Ovsynch组和PGF组出现新卵泡波的时间比CIDR组更早（p<0.05）。CIDR组排卵卵泡的平均直径小于Ovsynch组和PGF组（p<0.05）。CIDR组的排卵率为100%；Ovsynch组为75%；PGF组为70%。对照组的妊娠率（20%）低于CIDR组（35%）、Ovsynch组（40%）和PGF组（35%）。CIDR组怀孕小母牛的孕酮浓度在数值上更高，但与Ovsynch组、PGF组和对照组相比差异不显著（p>0.05）。
EAZI-BREED CIDR、基于Ovsynch的TAI和PGF方案在同步发情方面有效，并且在埃及水牛小母牛中导致了近乎相似的妊娠率。
