Samir Haney, Kandiel Mohamed M M, El-Maaty Amal M Abo, Sediqyar Manila, Sasaki Kazuaki, Watanabe Gen
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobeya, Egypt.
J Reprod Dev. 2019 Oct 23;65(5):451-457. doi: 10.1262/jrd.2019-035. Epub 2019 Aug 10.
This study aimed at investigating the efficacy of two protocols of estrous synchronization on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with controlled internal drug release (CIDR)-PGF2α (7-days CIDR insert with PGF2α injected on the 6 day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18). Blood sampling and ovarian ultrasound examinations (Grayscale, color and power Doppler modes) were conducted on the Day of CIDR removal, estrus, and luteal phase. Mean follicle diameter (MFD), first (1-LF) and second (2-LF) largest follicle diameters, and E2 levels significantly increased in the CIDR-PGF2α group at CIDR withdrawal. Ovsynch-CIDR markedly fortified higher follicle population, MFD, and 1-LF diameter at estrus and corpus luteum (CL) volume at the luteal phase in concomitant with increases (P < 0.05) in E2 (at estrus) and P4 (at luteal phase). At CIDR removal, the blue pixels in the dominant follicle (DF) were higher (1.5 times; P = 0.054) in the Ovsynch-CIDR than in the CIDR-PGF2α. At estrus, total blood flow (TBF) and power Doppler pixels (PDP) to DF(s) were noticeably higher (seven and 1.6 times; respectively) in the Ovsynch-CIDR than in CIDR-PGF2α (5906 ± 237 vs. 830 ± 60 pixels, P < 0.01 and 5479 ± 322 vs. 3377 ± 19 pixels, P < 0.05; respectively). At the luteal phase, TBF and PDP to the CL increased in the Ovsynch-CIDR group than in the CIDR-PGF2α group (11060 ± 965 vs. 7963 ± 480 pixels, 1.4 times, P = 0.05 and 18900 ± 1350 vs. 13220 ± 568 pixels, 1.1 times, P = 0.005; respectively). In conclusion, based on the improvement in synchronized follicular activity and hemodynamics, the Ovsynch-CIDR regimen is persuaded in Egyptian buffaloes.
本研究旨在调查两种发情同步方案对卵泡变化和血流动力学的效果。多胎埃及水牛（n = 36）采用可控性内部药物释放（CIDR）-PGF2α（7天的CIDR植入物，在第6天注射PGF2α；n = 18）或Ovsynch-CIDR（Ovsynch方案与7天的CIDR植入物同时使用；n = 18）进行同步发情处理。在去除CIDR当天、发情期和黄体期进行血液采样和卵巢超声检查（灰阶、彩色和能量多普勒模式）。在去除CIDR时，CIDR-PGF2α组的平均卵泡直径（MFD）、第一（1-LF）和第二（2-LF）大卵泡直径以及E2水平显著增加。Ovsynch-CIDR在发情期显著增强了较高的卵泡数量、MFD和1-LF直径，在黄体期增强了黄体（CL）体积，同时E2（发情期）和P4（黄体期）增加（P < 0.05）。在去除CIDR时，Ovsynch-CIDR组优势卵泡（DF）中的蓝色像素比CIDR-PGF2α组高（1.5倍；P = 0.054）。在发情期，Ovsynch-CIDR组中DF的总血流量（TBF）和能量多普勒像素（PDP）明显高于CIDR-PGF2α组（分别为7倍和1.6倍；5906 ± 237对830 ± 60像素，P < 0.01和5479 ± 322对3377 ± 19像素，P < 0.05）。在黄体期，Ovsynch-CIDR组中CL的TBF和PDP比CIDR-PGF2α组增加（11060 ± 965对7963 ± 480像素，1.4倍，P = 0.05和18900 ± 1350对13220 ± 568像素，1.1倍，P = 0.005）。总之，基于同步卵泡活动和血流动力学的改善，Ovsynch-CIDR方案在埃及水牛中具有优势。
