Shu Zhenbo, Chen Libo, Ding Dayong
Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, ChangChun, 130033, China.
Department of Ultrasound, China-Japan Union Hospital, Jilin University, ChangChun, 130033, China.
World J Surg Oncol. 2016 Sep 6;14(1):239. doi: 10.1186/s12957-016-0984-4.
microRNA (miRNAs) dysregulation is widely involved in cancer progression and contributed to sustained cell proliferation by directly targeting multiple targets. Therefore, better understand the underlying mechanism of miRNA in carcinogenesis may improve diagnostic and therapeutic strategies for malignancy.
We assessed microRNA-582 (miR-582-5P) expression in colorectal cancer (CRC) specimens and cell lines by real-time PCR. Luciferase reporter assay was used to confirm the target associations. Colony formation assay and anchorage-independent growth assay were used to analyze the effect of miR-582-5P on cell proliferation. Adenomatous polyposis coli (APC) gene and protein expression were examined using real-time PCR and western blotting, respectively.
miR-582-5P was upregulated in the CRC specimens and cell lines and targeted the 3' untranslated region of APC directly. miR-582-5P overexpression increased cyclin D1 and c-MYC expression, which subsequently induced CRC cell proliferation in an APC-dependent manner.
Our findings suggest that miR-582-5P plays an important role in the progression of CRC by inducing proliferation and may identify new targets for anti-cancer treatment.
微小RNA（miRNA）失调广泛参与癌症进展，并通过直接靶向多个靶点促进细胞持续增殖。因此，更好地了解miRNA在致癌过程中的潜在机制可能会改善恶性肿瘤的诊断和治疗策略。
我们通过实时PCR评估了结直肠癌（CRC）标本和细胞系中微小RNA-582（miR-582-5P）的表达。荧光素酶报告基因检测用于确认靶点关联。集落形成试验和非锚定依赖性生长试验用于分析miR-582-5P对细胞增殖的影响。分别使用实时PCR和蛋白质印迹法检测腺瘤性息肉病大肠杆菌（APC）基因和蛋白表达。
miR-582-5P在CRC标本和细胞系中上调，并直接靶向APC的3'非翻译区。miR-582-5P过表达增加了细胞周期蛋白D1和c-MYC的表达，随后以APC依赖的方式诱导CRC细胞增殖。
我们的研究结果表明，miR-582-5P通过诱导增殖在CRC进展中起重要作用，并可能为抗癌治疗确定新的靶点。
