Guo Aijiang
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu Province, People's Republic of China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu Province, People's Republic of China.
Parasit Vectors. 2016 Sep 5;9(1):490. doi: 10.1186/s13071-016-1782-0.
The cloacal tapeworm Cloacotaenia megalops (Hymenolepididae) is one of the most common cestode parasites of domestic and wild ducks worldwide. However, limited information is available regarding its epidemiology, biology, genetics and systematics. This study provides characterisation of the complete mitochondrial (mt) genome of C. megalops.
The complete mt genome of C. megalops was obtained by long PCR, sequenced and annotated.
The length of the entire mt genome of C. megalops is 13,887 bp; it contains 12 protein-coding, 2 ribosomal RNA and 22 transfer RNA genes, but lacks an atp8 gene. The mt gene arrangement of C. megalops is identical to that observed in Anoplocephala magna and A. perfoliata (Anoplocephalidae), Dipylidium caninum (Dipylidiidae) and Hymenolepis diminuta (Hymenolepididae), but differs from that reported in taeniids owing to the position shift between the tRNA (L1) and tRNA (S2) genes. The phylogenetic position of C. megalops was inferred using Maximum likelihood and Bayesian inference methods based on the concatenated amino acid data for 12 protein-coding genes. Phylogenetic trees showed that C. megalops is sister to Anoplocephala spp. (Anoplocephalidae) + Pseudanoplocephala crawfordi + Hymenolepis spp. (Hymenolepididae) indicating that the family Hymenolepididae is paraphyletic.
The complete mt genome of C. megalops is sequenced. Phylogenetic analyses provided an insight into the phylogenetic relationships among the families Anoplocephalidae, Hymenolepididae, Dipylidiidae and Taeniidae. This novel genomic information also provides the opportunity to develop useful genetic markers for studying the molecular epidemiology, biology, genetics and systematics of C. megalops.
泄殖腔绦虫巨头膜壳绦虫（膜壳科）是全球家鸭和野鸭中最常见的绦虫寄生虫之一。然而，关于其流行病学、生物学、遗传学和系统学的信息有限。本研究对巨头膜壳绦虫的完整线粒体（mt）基因组进行了特征分析。
通过长PCR获得巨头膜壳绦虫的完整mt基因组，进行测序和注释。
巨头膜壳绦虫的整个mt基因组长度为13,887 bp；它包含12个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因，但缺少一个atp8基因。巨头膜壳绦虫的mt基因排列与大裸头绦虫和叶状裸头绦虫（裸头科）、犬复孔绦虫（复孔科）以及微小膜壳绦虫（膜壳科）中观察到的相同，但与带绦虫科报道的不同，因为tRNA（L1）和tRNA（S2）基因之间的位置发生了偏移。基于12个蛋白质编码基因的串联氨基酸数据，使用最大似然法和贝叶斯推断法推断了巨头膜壳绦虫的系统发育位置。系统发育树显示，巨头膜壳绦虫是裸头绦虫属（裸头科）+克劳福德假裸头绦虫+膜壳绦虫属（膜壳科）的姐妹群，表明膜壳科是并系的。
对巨头膜壳绦虫的完整mt基因组进行了测序。系统发育分析深入了解了裸头科、膜壳科、复孔科和带绦虫科之间的系统发育关系。这一新颖的基因组信息也为开发有用的遗传标记提供了机会，用于研究巨头膜壳绦虫的分子流行病学、生物学、遗传学和系统学。
