Börsch G, Mai U, Reitemeyer E, Leverkus F, Opferkuch W
Medizinische Klinik Elisabeth-Krankenhauses Essen.
Immun Infekt. 1989 Jun;17(3):83-90.
The diagnostic performance of two different urease tests and of histologic search after modified Giemsa staining to detect Campylobacter pylori (CP) colonization of the upper gastrointestinal tract was prospectively investigated in 215 esophagogastroduodenoscopies, by using a sensitive culture technique as reference. Single antral urease tests had a high specificity of 95-96%, but a limited sensitivity of 78-83%, which increased to 91-94%, when one antral and one additional body biopsy were submitted to the biochemical tests. Giemsa stains were very sensitive, but less specific. The rate of colonization was similar in antrum and body biopsies, and increased with age. There was a close association of Campylobacter pylori colonization with duodenal and to a lower degree with gastric ulcer disease, but especially with gastritic mucosal changes. CP was never detected in patients without gastritis. Therefore, submitting one antral and one body biopsy specimen to validated urease tests represents a sensitive (91-94%) and specific (93%) method to detect Campylobacter pylori colonization, which appears to be a diffuse phenomenon affecting antral and body mucosae with similar frequency.
采用敏感培养技术作为参照，对215例食管胃十二指肠镜检查进行前瞻性研究，以评估两种不同的尿素酶试验以及改良吉姆萨染色后组织学检查在上消化道检测幽门螺杆菌（CP）定植的诊断性能。单项胃窦尿素酶试验具有95% - 96%的高特异性，但敏感性有限，为78% - 83%，若将一份胃窦活检组织和一份额外的胃体活检组织进行生化检测，敏感性可提高至91% - 94%。吉姆萨染色非常敏感，但特异性较差。胃窦和胃体活检组织中的定植率相似，且随年龄增长而增加。幽门螺杆菌定植与十二指肠溃疡密切相关，与胃溃疡的相关性较低，但与胃炎黏膜改变尤其相关。在无胃炎的患者中从未检测到CP。因此，将一份胃窦和一份胃体活检标本进行经过验证的尿素酶试验，是检测幽门螺杆菌定植的一种敏感（91% - 94%）且特异（93%）的方法，幽门螺杆菌定植似乎是一种以相似频率影响胃窦和胃体黏膜的弥漫性现象。
