Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture &Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Informatics, Huazhong Agricultural University, Wuhan 430070, PR China.
Sci Rep. 2016 Sep 6;6:32783. doi: 10.1038/srep32783.
A growing number of reports have revealed that microRNAs (miRNAs) play critical roles in spermatogenesis. Our previous study showed that miR-762 is differentially expressed in immature and mature testes of Large White boars. Our present data shows that miR-762 directly binds the 3' untranslated region (3'UTR) of ring finger protein 4 (RNF4) and down-regulates RNF4 expression. A single nucleotide polymorphism (SNP) in the RNF4 3'UTR that is significantly associated with porcine sperm quality traits leads to a change in the miR-762 binding ability. Moreover, miR-762 promotes the proliferation of and inhibits apoptosis in porcine immature Sertoli cells, partly by accelerating DNA damage repair and by reducing androgen receptor (AR) expression. Taken together, these findings suggest that miR-762 may play a role in pig spermatogenesis by regulating immature Sertoli cell growth.
越来越多的报道表明，microRNAs（miRNAs）在精子发生中发挥着关键作用。我们之前的研究表明，miR-762 在大白猪未成熟和成熟睾丸中表达差异。我们目前的数据显示，miR-762 直接结合环指蛋白 4（RNF4）的 3'非翻译区（3'UTR）并下调 RNF4 的表达。RNF4 3'UTR 中的一个单核苷酸多态性（SNP）与猪精子质量性状显著相关，导致 miR-762 结合能力发生变化。此外，miR-762 通过加速 DNA 损伤修复和降低雄激素受体（AR）表达，促进猪未成熟支持细胞的增殖并抑制其凋亡。综上所述，这些发现表明，miR-762 可能通过调节未成熟支持细胞的生长在猪精子发生中发挥作用。
