Gallo Juliana Failde, Pinhata Juliana Maira Watanabe, Chimara Erica, Gonçalves Maria Gisele, Fukasawa Lucila Okuyama, Oliveira Rosangela Siqueira de
Instituto Adolfo Lutz, Centro de Bacteriologia, Núcleo de Tuberculose e Micobacterioses, São Paulo, SP, Brasil.
Instituto Adolfo Lutz, Centro de Imunologia, Laboratório de Diagnóstico Molecular de Infecções Bacterianas, São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 2016 Sep;111(9):545-50. doi: 10.1590/0074-02760160048. Epub 2016 Sep 1.
Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
巴西是结核病高负担国家之一，快速诊断对于有效控制该疾病至关重要。在本研究中，在一家参考实验室的常规诊断条件下，对一种针对mpt64基因以鉴定结核分枝杆菌复合群分离株的内部实时聚合酶链反应（PCR）检测方法进行了评估。2011年5月至2012年7月，前瞻性地提交了1520株分枝杆菌分离株进行表型和/或PRA-hsp65鉴定以及实时PCR检测。mpt64实时PCR显示出99.7%的敏感性和96%的特异性，检测出了79.4%通过表型和PRA-hsp65鉴定漏检的病例。该内部实时PCR检测方法显示出高敏感性和特异性，并成功应用于来自高负担地区的一家参考实验室的结核病常规诊断。
