Starnes J W, Cantu G, Farrar R P, Kehrer J P
Department of Kinesiology, University of Texas, Austin 78712.
J Appl Physiol (1985). 1989 Jul;67(1):69-75. doi: 10.1152/jappl.1989.67.1.69.
The effects of chronic endurance exercise and food restriction on nonenzymatic lipid peroxidation (LP) of gastrocnemius muscle during aging were studied in male, Fischer 344 rats. One set of rats aged 6 and 18 mo were assigned to an exercise group (treadmill running) or an age-matched sedentary control group. After 6 mo (at the ages of 12 and 24 mo), LP and levels of alpha-tocopherol and its oxidized form, alpha-tocopheryl quinone, were measured. The extent of LP was determined in homogenates by measuring the content of thiobarbituric acid-reactive substances. After homogenization, the muscles were immediately evaluated for basal LP and also incubated in the presence of oxidant stressors for 2 h to assess antioxidant capacity (AOC) and for 24 h to estimate total peroxidizable lipid (TPL). Basal LP was not affected by age or exercise. AOC was not affected by exercise at either age. However aging significantly decreased AOC and increased alpha-tocopheryl quinone in both sedentary and exercised groups. TPL was not affected by age, but was increased by exercise training (P less than 0.05). Another set of rats was divided into the following three groups at 3 mo of age: sedentary, fed ad libitum (S); sedentary, caloric restricted by alternate day feeding (R); and exercised by forced treadmill running (E). Two years later, when the rats were 27 mo of age, the extent of LP was assessed.(ABSTRACT TRUNCATED AT 250 WORDS)
在雄性Fischer 344大鼠中研究了长期耐力运动和食物限制对衰老过程中腓肠肌非酶促脂质过氧化（LP）的影响。将一组6个月和18个月大的大鼠分为运动组（跑步机跑步）或年龄匹配的久坐对照组。6个月后（12个月和24个月龄时），测量LP以及α-生育酚及其氧化形式α-生育醌的水平。通过测量硫代巴比妥酸反应性物质的含量来测定匀浆中LP的程度。匀浆后，立即评估肌肉的基础LP，并在氧化应激源存在下孵育2小时以评估抗氧化能力（AOC），孵育24小时以估计总可过氧化脂质（TPL）。基础LP不受年龄或运动的影响。两个年龄组的AOC均不受运动的影响。然而，衰老显著降低了久坐组和运动组的AOC，并增加了α-生育醌。TPL不受年龄影响，但运动训练使其增加（P<0.05）。另一组大鼠在3个月大时分为以下三组：久坐、自由进食（S）；久坐、隔日喂食热量限制（R）；以及强制跑步机跑步运动（E）。两年后，当大鼠27个月大时，评估LP的程度。（摘要截断于250字）
