牛感染大肠杆菌 O104:H4 暴发菌株的实验。
Experimental Infection of Calves with Escherichia coli O104:H4 outbreak strain.
机构信息
Friedrich-Loeffler-Institut, Institute of Molecular Pathogenesis, Naumburger Str. 96a, 07743 Jena, Germany.
Friedrich-Loeffler-Institut, Department of Experimental Animal Facilities and Biorisk Management, Südufer 10, 17493 Greifswald - Insel Riems, Germany.
出版信息
Sci Rep. 2016 Sep 7;6:32812. doi: 10.1038/srep32812.
In 2011, a severe outbreak of hemolytic-uremic syndrome was caused by an unusual, highly virulent enterohemorrhagic E. coli (EHEC) O104:H4 strain, which possessed EHEC virulence traits in the genetic background of human-adapted enteroaggregative E. coli. To determine magnitude of fecal shedding and site of colonization of EHEC O104:H4 in a livestock host, 30 (ten/strain) weaned calves were inoculated with 10(10) CFU of EHEC O104:H4, EHEC O157:H7 (positive control) or E. coli strain 123 (negative control) and necropsied (4 or 28 d.p.i.). E. coli O157:H7 was recovered until 28 d.p.i. and O104:H4 until 24 d.p.i. At 4 d.p.i., EHEC O104:H4 was isolated from intestinal content and detected associated with the intestinal mucosa. These results are the first evidence that cattle, the most important EHEC reservoir, can also carry unusual EHEC strains at least transiently, questioning our current understanding of the molecular basis of host adaptation of this important E. coli pathovar.
2011 年，由一种不寻常的、高毒力肠出血性大肠杆菌（EHEC）O104：H4 菌株引起了严重的溶血性尿毒综合征爆发，该菌株在适应人类的肠聚集性大肠杆菌的遗传背景中具有 EHEC 毒力特征。为了确定 EHEC O104：H4 在牲畜宿主中的粪便脱落量和定植部位，将 30 只（每株 10 只）断奶小牛用 10（10）CFU 的 EHEC O104：H4、EHEC O157：H7（阳性对照）或大肠杆菌 123 株（阴性对照）接种，并进行剖检（4 或 28 d.p.i.）。EHEC O157：H7 一直被回收至 28 d.p.i.，而 O104：H4 则持续至 24 d.p.i.。在 4 d.p.i.时，从肠内容物中分离出 EHEC O104：H4 并检测到与肠黏膜相关。这些结果首次证明，牛作为最重要的 EHEC 储存宿主，至少可以暂时携带不寻常的 EHEC 菌株，这对我们目前对这种重要大肠杆菌血清型的宿主适应性的分子基础的理解提出了质疑。
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