Liu Wanbo, Chen Sheng, Liu Bing
Department of Medical Affairs, The General Hospital of the PLA Rocket Force, Beijing, China.
Department of General Surgery, The General Hospital of the PLA Rocket Force, Beijing, China.
Pediatr Surg Int. 2016 Nov;32(11):1059-1065. doi: 10.1007/s00383-016-3960-8. Epub 2016 Sep 6.
Hepatoblastoma (HB) is the most common primary malignant tumor of the liver in young children. The aim of this study is to identify the diagnostic and prognostic values of serum exosomal miR-21 in Chinese patients with HB.
We retrospectively reviewed 32 children with HB. The expressions of miR-21 were detected by real-time PCR. The comparison of diagnostic performance of plasmatic, exosomal miR-21 and AFP levels was measured using the Area Under ROC Curve.
For patients in HB group, miR-21 concentration was significantly higher in the exosomes compared with the exosome-depleted supernatants and whole plasma. Expression of miR-21 was significantly higher in patients with HB compared with control group in both plasma and exosomes. With respect to the diagnosis of patients with HB, exosomal miR-21 was significantly more accurate compared with the Alpha-fetoprotein levels. Moreover, exosomal miR-21 was an independent predictor of Even-free survival for patients with HB.
In this study, we found that expression of miR-21 was significantly higher in patients with HB compared with control group in both plasma and exosomes, and we confirmed that exosomal miR-21 could be defined as a diagnostic and prognostic biomarker for patients with HB.
肝母细胞瘤（HB）是幼儿最常见的原发性肝脏恶性肿瘤。本研究旨在确定血清外泌体miR-21在中国HB患者中的诊断和预后价值。
我们回顾性分析了32例HB患儿。采用实时PCR检测miR-21的表达。使用ROC曲线下面积测量血浆、外泌体miR-21和甲胎蛋白水平的诊断性能比较。
对于HB组患者，与去除外泌体的上清液和全血浆相比，外泌体中miR-21浓度显著更高。在血浆和外泌体中，HB患者的miR-21表达均显著高于对照组。关于HB患者的诊断，外泌体miR-21比甲胎蛋白水平显著更准确。此外，外泌体miR-21是HB患者无事件生存期的独立预测指标。
在本研究中，我们发现血浆和外泌体中HB患者的miR-21表达均显著高于对照组，并且我们证实外泌体miR-21可被定义为HB患者的诊断和预后生物标志物。
