Ascalone V, Dal Bo L, Deschamps N, Guinebault P
Pharmacokinetic Laboratory, LERS Synthelabo, Limito-Milan, Italy.
J Chromatogr. 1989 May 5;490(1):81-90. doi: 10.1016/s0378-4347(00)82763-2.
A sensitive capillary gas chromatographic method was developed for the determination of fengabine (a GABAergic antidepressant drug) and some of its metabolites in plasma samples. The method involves a single and rapid liquid-liquid extraction of the parent drug and metabolites from plasma buffered at pH 5, evaporation of the organic phase under nitrogen, derivatization to tert.-butyldimethylsilyl ethers and esters and automatic gas chromatography on a fused-silica, silicone-bonded capillary column coupled to an electron-capture detector. The detection limit for fengabine and other compounds is lower than 1 ng/ml in plasma; the method was successfully applied to pharmacokinetic and drug monitoring clinical studies and tested on more than 2000 biological samples and was found not to suffer from endogenous or exogenous interferences.
建立了一种灵敏的毛细管气相色谱法，用于测定血浆样品中加巴喷丁（一种γ-氨基丁酸能抗抑郁药物）及其某些代谢物。该方法包括从pH 5缓冲的血浆中单次快速液液萃取母体药物和代谢物，氮气下蒸发有机相，衍生化为叔丁基二甲基甲硅烷基醚和酯，以及在与电子捕获检测器相连的熔融石英、硅键合毛细管柱上进行自动气相色谱分析。加巴喷丁和其他化合物在血浆中的检测限低于1 ng/ml；该方法已成功应用于药代动力学和药物监测临床研究，并在2000多个生物样品上进行了测试，发现不存在内源性或外源性干扰。
