Le Normand Y, De Villepoix C, Athouel A, Kergueris M F, Bourin M, Larousse C
Département de Pharmacologie, Faculté de Médecine, Nantes, France.
Fundam Clin Pharmacol. 1988;2(6):551-8. doi: 10.1111/j.1472-8206.1988.tb00655.x.
A gas chromatographic method for routine quantitation of methohexital in plasma samples is reported. One-step extraction in organic phase, the use of a fused silica capillary column, and nitrogen-selective detection permit simple, precise, and sensitive determination of methohexital in plasma. A linear relationship is described between peak height ratio and methohexital concentrations ranging from 0.125 to 50.0 micrograms/ml (r = 0.998). The sensitivity limit of the assay was 6 ng/ml in plasma. No interfering peak was observed with numerous other drugs. The procedure was successfully applied to the determination of pharmacokinetic parameters of methohexital after IV administration or continuous infusion in a child and an adult.
报道了一种用于常规定量血浆样品中美索比妥的气相色谱方法。有机相一步萃取、使用熔融石英毛细管柱以及氮选择性检测，使得能够简单、精确且灵敏地测定血浆中的美索比妥。峰高比与美索比妥浓度在0.125至50.0微克/毫升范围内呈线性关系（r = 0.998）。该测定法在血浆中的灵敏度极限为6纳克/毫升。未观察到与众多其他药物的干扰峰。该方法成功应用于一名儿童和一名成人静脉注射或持续输注美索比妥后的药代动力学参数测定。
