Fortune N S, Geeves M A, Ranatunga K W
Department of Biochemistry, University of Bristol, School of Medical Sciences, U.K.
J Muscle Res Cell Motil. 1989 Apr;10(2):113-23. doi: 10.1007/BF01739967.
The effect of changes in hydrostatic pressure (up to 10 MPa) on the maximally calcium-activated tension in glycerinated rabbit psoas fibres has been examined. The steady active tension was depressed by 0.8% per MPa pressure rise. This pressure sensitivity was enhanced by the pressure of millimolar phosphate and depressed by millimolar ADP. These results support the conclusions that increased pressure is perturbing a crossbridge event. The results are discussed in terms of a three state crossbridge model and are shown to be compatible with a pressure effect on the transition from an attached crossbridge state to a tension bearing state. This is compatible with the effects of pressure on the isolated proteins in solution.
